伏马毒素B1新型荧光标记物制备及其在荧光偏振分析技术中的应用.pdf

第6卷第12期
食品安全质量检测学报
Vol 6 No 12
2015年12月
Journal of Food Salcly and Quality
IDcc..2015
伏马毒素B1新型荧光标记物制备及其在
荧光偏振分析技术中的应用
刘继红23, Chris Maragos4,王敏2,3,尹海燕,2,张玲23,
张军锋,洪慧杰,2,3,王红旗12,3
(1.河南省农业科学院农业质量标准与检测技术研究所.郑州4500022.河南省粮食质量安全与检测重点实验室
郑州450002:3.农业部农产品质量安全风险评估实验室,郑州4500024.美国农业部(USDA)
国家农业应用研究中心( NCAUR)食源性病原菌与真菌毒素研究分部,皮奥里亚61604)
摘要:目的针对玉米中常见真菌毒素一伏马毒素131(131)开发新型荧光标记物,从而替代昂贵的6-(4,6-二
氯三嗪基)氨基荧光素(6-DIAT)标记物,并在此基础上开发基于荧光偏振原理的伏马毒素快速检测技术。方法
采用一锅煮的合成方法,以5-(4.6-二氯三嗪基)氨基荧光素(5-IAP)为荧光基团制备伏马毒素荧光标记物,然后
利用荧光偏振的原理建立基于5-IIAF的伏马毒素快速检测技术。结果基于S-IIAF的131荧光标记物与基于
6-DTAF的IB1荧光标记物的荧光偏振分析方法具有相同的分析性能,TCs值基本相等。结论采用S-DTAT成
功制备了HB荧光标记物,同时利用该荧光标记物开发了伏马毒素荧光偏振快速检测技术。该分析技术与基于
6-IDTA荧光标记物的TB1荧光偏振速測技术相比,具有同样的分析性能,但分析成本降低了一半。
关键词:伏马毒素B;氨基荧光素:荧光标记物;荧光偏振
Preparation and application of new fluorescein-labeled fumonisins B in
fluorescence polarization analysis technigue
LIU Ji-hong*, Chris Maragos, WANG Min", YIN Hai-yan,, ZHANG Ling
ZHANG Jun-feng"-, HONG Hui-jie", WANG Hong-qi
(1. nstitute of uality Standad and esting technolo) fora870-produer leman Academy of icu un a/ Science,
Zhengzhon 450002, hina; 2. Hen Key Labo ory Gin aliy and Safey and Testing, Zhengzhou450002
Chin; 3. Laborato o/Quality Sa/et Risk esme or duc s(hen zhou),Ministro.
Zhengzhou 450002, China; 4. Bacteria/ Foodborne Pathogens and Mycology Research Unit, Peoria IL 61604, USA)
ABSTRACT: Objective To Prepare a new fluorescent tracer against common mycotoxins such as
umonisins B [or replace 6-(4, 6-dichlorolriazinyl)aminofluorescein(6-dtaf)expensive marker, and to
develop a technique for quick detection of fumonisins B based on the principle of fluorescence polarization
Methods The onc-pot method was used to synthesiz 5-(4,6-dichlorotria7iny1)aminofluorescein (5-DTAP
基金顼目:农业部重点项目(2011-G5)、粮油作物产品未知危害因子识别与已知危害因子安全性评估(GJHP2015007)、河南省农业科学院
优秀青年科技基金(2013YQ24)
Fund: Supported by the Kcy Roject of the Ministry of Agriculture(2011-g5), Projcet of Unknown Hazards Factor Idcntification and the Known
Factor Safety Asscssment for Iroducts of Grain and Oil Crops(GJFP2015007) and Scicncc Technology Foundation for Outstandin
Young Scientists of I lenan Academy of A gricultural Sciences (2013YQ2
通讯作者:王红旗,副研究员,主要研究方向为农产品质量安全检测技术。T-mal:huda2000c0126.com
Corresponding author: WANG Hong-qi, Associate Rcscarchcr, Institute of Quality Standard and Testing Tcchnology for Agro-products
HcnanacademyofAgrculturalScicncc,No.116,Huayuanroad,JinshuiDistrict,Zhengzhou450002,China.e-mail:huda20000126.com