稀土-荧光素双重荧光响应pH探针分子的合成与荧光性质研究.pdf

第27卷第10期
无机化学
报
Vol27 No10
2011年10月
CHINESE JOURNAL OF INORGANIC CHEMISTRY
2071-2075
稀土ー荧光素双重荧光响应pH探针分子的合成与荧光性质研究
景旭1刘福臣*1张晓琳2何成*2
(天津理工大学化学化工学院,天津300384)
(大连理工大学化工学院,精细化エ国家重点实验室,大连116012)
摘要:将含有8-氨基喹啉与荧光素行生物缩合的席夫碱QZ1与销⑩的TA(噻昐基三氟乙酰丙酮化合物作为基本原料合成了
新的销⑩-荧光素化合物Eu-QZ1,并对其进行了光谱表征。化合物Eu-QZ1在470nm光的激发下产生荧光素的特征绿色荧光
(530mm)。在从碱到酸(pH从9.0到6)滴定过程中该荧光的强度增强了接近5倍,其pK,值计算为7.30。在370m光的激发
下,Eu,QZ1发射稀土销Ⅷ离子的特征红色荧光(主峰位于612mい。在从酸到碱H从55到85)滴定过程中,该荧光的强度增强
了接近10倍,其pK。值计算为7.39。这些结果表明Bu-Q21是1个高灵敏度双荧光响应的pH探针。细胞试蛻表明FEu-QZ1具有
良好的细胞通透性,能够在红光和绿光两个位置标记海拉细胞中的pH范围。
关键词:荧光素;铕离子;pH荧光探针;细胞成像
中图分类号:0614.33文献标识码;A文章编号:1001-4861(2011)10-2071-05
Synthesis and Fluorescent Study on Europium(D-fluorescein
Compound as a Dual-responding Fluorescent ph Sensor
JING Xu LIU Fu-che* ZHANG Xiao-lin HE Cheng*2
School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianijn 300384, China)
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning 116012, China)
Abstract A new europium(ID compound EU-QZI was designed and synthesized as a ph-sensitive fluorescent
probe ( where QZ1=()-2-(3-((u n i 8 mn) m h )-6h-xanthen-9- )benzoic acid). EU-QZ1
comprised a green light emitins fluorescencein fluorophore and a Eu moiety as the origin of red luminescence
These ph-sensitive emitter components have pk, values at about 7.07.4. Luminescence titrations demonstrate
that EU-QZ1 exhibit well sensitivity in monitoring PH value changes in neutral aqueous media and is able to
respond PH value in cultured cells
Key word: fluorescein; Eu], PH fluorescent probe; cell imaging
Protons are one of the most important targets PH probes have been devised". Since minor variations
among the intracellular species, due to that the proton of PH value may induce cellular dysfunction, desirable
concentration, as an important metabolic and cellular ph fluorescent probes should respond sensitively to the
parameter, plays a centre role in many cellular events La)
minor
PH value deviation, while avoiding the
The fluorescent PH probe for intracellular PH imaging interference from other intracellular species-
is currently intriguing the attention of chemists, despit
Lanthanide are elements of growing importance
several different approaches to biological luminescence due to their unique optical properties arising from the f-
收稿日期:2011-01-04。收修改稿日期:2011-04-27
国家自然科学基金资助项目(No.20801041)。
*通讯联系人。E-mal:fuchenliutj@yahoo.com,hecheng@dlut.edu.cn