食品中马来酸的高压液相色谱法检测研究.pdf

第4卷第5期
食品安全质量检测学报
2013年10月
Journal of Pood Safely and Quality
Oct.,2013
食品中马来酸的高压液相色谱法检測研究
陈健,林真2,张玉燕,钱疆,蔡少立,杨方"
(1.福建出入境检验检疫局检验检疫技术研究重点实验室,福州350001
2.福建生物工程职业技术学院保健营养学系,福州350002)
摘要:目的探讨应用高压液相色谱法来检测各种食品中的马来酸。方法以 Agilent SB C1s色谱柱为分析
柱,确立了对淀粉、改性淀粉、蛋酥饼干、芋泥汤圆、刀削面、粉丝等食品中马来酸的高压液相色谱检测法。
通过试验,考察了富马酸对这些食品中的马来酸检測的影响。结果在试验条件下,富马酸不会对马来酸的检
测造成影响。通过对方法的回收率和精密度进行分析探讨,结果在所确定的实验条件下,即流动相:0.1mol/L
磷酸二氢铵溶液pH调至2.5,流速:0.6mL/mnin,检测波长:240m,柱温:30℃,峰面积和标准溶液在0.5
20.0mgL范围内呈良好线性关系,线性系数大于0.990平均回收率处于93.8%~101.1%,RSD均小于5.0%
结论本实验方法安全可靠,可以满足实际检测的需要
关键词:高压液相色谱法;马来酸;富马酸;食品;淀粉
Research on determination of maleic acid in food by high pressure
liquid chromatography
CHEN Jian, LIN Zhen,, ZHANG Yu-yan, QIAN Jiang CAL Shao-li, YANG Fang
(1. Fujian Einlry-exil inspection and Quarantine Bureau, Fuzhou 350001. China
2. Health Department of Fujian Technology Biological Engineering Institate, Fuzhou 350002, China)
ABSTRACT: Objective To establish a high pressure liquid chromatography (HPLC)method for determina
tion of maleic acid in starch, modified starch, egg biscuit and some foods. Methods With Agilent Zorbax
Stabond Cl8 column, the effect of the fumaric acid was studied. Results The peak area and the standard solu
tion in the 0. 5-20 mg/L kept a good linear relationship, with a correlation coefficient of more than 0.999. The
extraction recoveries were between 93.. 1%% and the RSD was less than 5.0% Conclusion This labor-
atory method can meet the requirement for determination in practice
KEY WORDS: high pressure iquid chromatography: maleic acid; fumaric acid; food; starch
2013年5月15日,据中国台湾“今日新闻网”“检出顺丁烯二酸产品及其原料封存回收统计表
报道,嘉义县调查站3月接获检举食物含毒淀粉顺目前已累计查获回收违规产品和原料692.05吨,此
丁烯二酸酐,卫生部门随即展开稽查,截至6月中事件成为台湾地区“塑化剂事件”后的又一重大食品
旬,根据台湾地区“卫生署食药局”在其官网公布的安全事故。
基金项目:十ニ五科技计划项目(2011BAK10BO4)、福建省科技项目(2012Y6001
I und: Supporled by the 12th Five-year plan (201 1 BAK10B04) Fujian province science and technology project (2012Y600 1
通讯作者:杨方,研究员,主要研究方向为食品理化分析检测。E-mail:yftjciq(G@gmail.com
Corresponding author: YANG Ian, Professor, Technical Center of Fujian Entr -Exit Inspection &Quarantine Bureau, No31, Luong Road,
Fuzhou350001.China.Email:yffjciq(@gmail.com