固定化酶催化椰子油水解工艺研究.pdf

CHINA OILS AND FATS
2016Vol.41No.1
油脂化工
固定化酶催化椰子油水解工艺研究
叶棋锋',王挥,赵松林,陈卫军,王兴国
(1.江南大学食品学院,江苏无锡214122:2.中国热带农业科学院椰子研究所,
海南文昌571339:3.海南大学,海口570228)
摘要∷利用水浴振荡辅助固定化酶进行椰子油水解工艺研究。比较3种固定化酶水解效率并筛选。
以反应体系的酸值为响应值,以温度、pH、加酶量、水油质量比为影响因素,采用单因素实验结合
Box- Benken实验,建立反应体系酸值的二次多项式回归方程,通过响应面分析椰子油的最佳固
定化酶水解条件,并在优化条件下研究酶的回收及操作稳定性。结果表明,温度80℃、pH5.5、I
pomme435加酶量6.51%和水油质量比1.08:1为固定化酶催化椰子油水解的最佳工艺条件,该条
件下反应20h,椰子油的水解率为94.80%,重复使用10次后,酶活仍能保留86%,稳定性较好。
关键词∵固定化酶椰子油水解;响应面
中图分类号:TQ645:7Q426
文献标识码∵A
文章编号:1003-7969(2016)11-0036-05
Hydrolysis of coconut oil catalyzed by immobilized lipase
YE Qifeng", WANG Hui, ZHAO Songlin
CHEN Weijun, WANG Xingguo
(1. College of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2. Coconut Research Institute, Chinese Academy of Tropical Agriculture Sciences, Wenchang 571339
Hainan, China: 3. Hainan University, Haikou 570228, China
Abstract In water bath oscillator, the hydrolysis of coconut oil catalyzed by immobilized lipase was stud
ied. The hydrolysis effects of three kinds of immobilized lipases were compared and the best immobilized
lipase was screened. With acid value of reaction system as response value, and temperature, PH, dosage of
enzyme and mass ratio of water to oil as factors, quadratic polynomial regression equation of acid val
was established by single factor experiment and Box-behnken experiment, and the hydrolysis conditions of
coconut oil were optimized by response suface methodology. Also the recycling and operation stability of
the lipase were studied under the optimal conditions. The results showed that the optimal hydrolysis condi
tions of coconut oil were obtained as follows temperature 80 C, ph 5. 5, dosage of Lipozyme 435 6.51%
and mass ratio of water to oil 1.08: 1. Under these conditions, the hydrolysis rate was.80% after reaction
for 20 h and the enzyme activity retained 86 after ten times recycling, showing a good stability
Key words immobilized lipase, coconut oil hydrolysis, response surface methodology
椰子油是由椰肉压榨制得,脂肪酸组成特殊,富52%,具有快速补充能量、抗菌、抗病毒、抗氧化
含中碳链脂肪酸,其中辛酸含量为4.6%~10%,癸降血脂、降胆固醇等功效町。椰子油与棕榈仁油并
酸含量为5.5%~8.0%,月桂酸含量为45%
称为自然界中制备中碳链脂肪酸最丰富的原料
来源。
收稿日期2015-12-29:修回日期:2016-06-2
基金项目:海南省自然科学基金(20153166
油脂水解是制备脂肪酸的主要途径,传统的油
作者简介:叶棋锋(1990,男,硕士研究生,研究方向为油脂脂水解方法主要有常压酸解法、皂化酸解法、加压水
化学(Emai)565480644@qq.com
解法、碱解法等,然而这些水解方法通常需要添
通信作者王兴国,教授(E-mai)wxg1002@q.com。
加酸、碱、金属氧化物等催化剂或需要高温、中高压
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