金属络合法纯化火炭母黄酮工艺研究.pdf

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金属络合法纯化火炭母黄酮工艺研究
董艳辉
(び东约学院医约化T学院,中山524858)
摘要:月的:研究金属络合法纯化火炭母黄酮的Tど。方法:对4种不同金属盐与火炭母黄酮的络合
效果进行了比较,并通过单因索实验优化金属络合法纯化火炭母黄的最工艺。结果:筛选出最仹络合金
属盐为氯化钙,得到金属络合法纯化火炭母黄酮的最仕工艺条件为:反应液的pH为8.0、氯化钙溶液浓度为
6.0 ammo/L、輔浓度为0.2mgmL,解络剂EDTA与络合剂CaC12的摩尔比为1.5:1。在此纯化条件下,
含量由粗提物的20.5%增加到56.5%,提高了2.8倍。结论:采用金属络合法纯化火炭母总是一种简单
有效的纯化方淞。
关键词:火炭母,黄酮;金属络个;纯化
中图分类号:R284.2
文献标识码:A
文章编号:1006-2513(2015)01-0138-05
Purification of flavonoid from polygonum Chinense
by metal chelating method
DONG Yan-hui
(School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458
Abstracts: Ob]clive: The purilicalion of lavonoid from polygonum Chinense by mctal chelating method was
studied. Methods: Four diffcrent kinds of metal salls were tested on the chelating capacity with lavonoid. The optimal
process for the purification of flavonoid was explored on the basis of single factor experiments. Results: The best metal
sall was calcium chloride. The optimum conditions of purification were as follows: pll valuc 8.0, calcium chloride
solution concentration 6.0 mmoll, flavonoid concentration 0.2 mg/ml, and mole ratio of ED IA as decomposing
chelating agent and calcium choride 1.5 1. Under the above conditions, the contents of lavonoid was increased
From 20.5% to 56.2% with 2.8 times as higher as the crude extracts. Conclusion: Purilication of flavonoid Polygonum
Chinense by metal chelating method was simple and effective
Key words: polygonum Chinense: lavonoid meal complex: purification
火炭母为夢科(Po/ gonacece)蓼属植物火炭黄酮类、鞣质类、醇类等P-。韦等对不同
( Polygonum Chinense L.)的干燥个草,具有产地火炭母的总黄含量进行了测定,发现火炭
清热利湿、凉血解毒、平肝明门、活血通络等功母含有丰富的黄酮。黄啊类化合物是一类重要
效,是岭陌地区常用的一味中草药,也足多种涼的中药成分,具有多种多样的生物活性,一方師
茶如“广东凉茶”、“工老吉”及“二十四味”等通过其清除白山基的作用,直接抑制癌细胞的牛
的主要组成药物之?。火炭母主要化学成分为长、从而提高机体免疫力面实现抗癌、防癌的作
收稿日期:20140917
作者简介:並艳辉(1981-),女(汉族),硕十研究4,实验师,研究方向:现代分析技术及应用。L-mail:dyh810226a126.com。
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