金属络合法纯化玉米须黄酮工艺研究.pdf

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金属络合法纯化玉米须黄酮工艺研究
常影,焦岩,宫春宇,于笛,韩易
(1.齐齐哈尔大学食品与生物工程学院,齐齐哈尔161006;
2.并齐哈尔大学农产品加工黑龙江省普通高校亜点实验卒,齐齐哈尔161006
摘要:貫类化合物的纯度是影响峒类化合物发挥其牛物活性的关键所在。为了寻找一种方便快捷、
成本低、效果好?有利」保护黄酮类化个物活性的纯化黄酮的方法,本文以玉米须提取物为研究村象,采用
金属络合法研究了正米须貞同的纯化工艺,通过単因素试验研究了不同質躺浓度、金属络合时间、乙酸锌浓
度、pH和 FDA用量对纯化效果的影响,并通过止交试验优化得到金属络合法纯化玉米须黄酮的最佳⊥艺
条件为:萸嗣浓度为0.045mgmL,络合时间25min,乙酸锌浴液浓度6.5 mmol/L,反应液pH为8.5,解络剂
FDTA川量为2.5gg黄酮。在此纯化条件下,下米须黄弱纯度巾粗提物的3.02%增加到35.32%,是高了10.7
倍。实验结果表明,金属络合法是?种简単、纯化效果好、价格低耗的纯化玉米须萸両的方法,具有实际应用
前景。
关键词:下米须;萸酮;金属络合;纯化
中图分类号:TS202.1
文献标识码
文章编号:10062513(2015)01004505
Purification of corn silk flavonoids by metal chelation method
CHAN Ying, JIAO Yan, GONG Chun-yu, YU Di, HAN Yi
(1. Collcgc of Food and Biological Enginccring, Qiqihar University, Qiqihar 161006:
2. Key Laboratory of Processing, Agricultural Products of Heilongjian Province, Qiqihar University, Qiqihar 161006
Abstract The purity of flavonoids have important influence on the biological activity. To develop a convenient and
fast, low cost and high biological aclivily lavonoid-purilied method, the process parameters of the purification of
corn silk flavonoids by meta chelation method were studied. The optimal process for the purification of flavonoids
was optimized on the basis of single factor and orthogonal test. The optimized parameters were as follow: flavonoids
concentration 45 H g/m., chelating time 25min, inc acetate solution concentration 6.mmo,T 10m., PH 8.5
IDTA 2. 5g/g flavonoids. After being purified, the purity was increased from 3.02%o to 35.32%o with 10.7 times
high as the crude extracts. The metal chelation flavonoid-purified method was feasible with convenience, higher
purification yield and better prospect
Key words: corn silk; flavonoids; mctal complcx; purification
玉米须又名玉麦须、玉米胡子、棒子毛。质放活性成分,如脂肪油、挥发油、苦味糖忒
除玉米粒外,玉米苞叶、玉米芯和玉米须等玉米隐黄素、维生素C、有机酸等,还含有较高药用
的副沪品都被人家所忽略,玉米须作为副产物仅价的皂苷、黄和生物碱类化合物等物质2习
被用作饲料。研究表明,床米须含有许多営养物现代药埋研究证明,米须有消肿利尿、清热解
收稿日期:2014-09-0
基金项目:国家自然科学基金项目(31301596)。
作者简介:常影(1982-),女,助理研究员,硕士,研究方向为植物化学。画讯作者:焦,lanyan_3000126.com。
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