反相高效液相色谱法同时测定黄豆酱中的苯甲酸、山梨酸、安赛蜜.pdf

中国食品添加剂
China Food Additives-
分析测试
反相高效液相色谱法同时测定黄豆酱中的
苯甲酸、山梨酸、安赛蜜
朱小梅12,黄柳霞,,廖权丰
(1.广东省食品质量监督检验站,广州510308;2.广东省食品工业研究所,广州510308;
3.广东省食品添加剂重点实验室,广州510308)
摘要:建立一种用反相高效液相色谱法同时测定黄豆酱中苯甲酸,山梨酸和安赛蜜的新方法。样
品经酸化后,用无水乙醚萃取,并浓缩吹干,再加入碳酸氯钠溶液溶解,样品调pH后,采用C18柱
(25mm×4.6mm,5um)分离,以甲醇(5%)+0.02moLL乙酸铵溶液(95%)为流动相,流速1.0 ml/min,
检测波长214nm(安赛蜜)及230nm(苯甲酸和山梨酸),出峰时间定性,外标法定量。结果表明:该方法的
检出限为 Img/kg,线性范围为1~50 ug/ML,相关系数分别为:安赛蜜R-0.9959;苯甲酸R=1.000山梨
酸R=0.99993。回收率分别为:安赛蜜89.4%~94.1%;苯甲酸97.6%~101%;山梨酸89.6%~93.2%。相
关标准偏差(RSD)分别为:安赛蜜0.38%~2.32%;苯甲酸0.26%ン1.04%;山梨酸0.44%~1.82%。该方
法准确方便,灵敏度高,可实现黄豆酱中苯甲酸、山梨酸、安赛蜜的测定。
关键词;:反相高效液相色谱法;苯甲酸;山梨酸;安赛蜜;黄豆酱
中图分类号:TS202.1文献标识码:A文章编号:10062513(2015)06019409
Determination of benzoic acid, sorbic acid and acesulfame potassium
in soya bean jam by RP -HPLC
ZHU Xi
ao-meil, 2
HUANG Liu-xia, LIAO Quan-feng, s
Guangdong Food Quality Supervision and Inspection Station Guangzhou 510308; 2.Guangdong Food
Industry Institute, Guangzhou 510308 3 Guangdong Provincial Key Laboratory of food additives,
Guangzhou 510308
Abstract: A novel mcthod is cstablish for the scparation and dctermination of bcnoic acid, sorbic acid and
sulfate potassium in soya bean jam by reversed phase high performance liquid chromatography(rp-hplc)
The samplc was acidified and cxtracted by anhydrous cthcr. Aftcr the cthcr was blown oul by nitrogcn, the residual
werc dissolved by sodium bicarbonate solution. Thc componcnts were analyzed by C18(250mmx4 6mm i.d., 5 m
after adjusting ph The mobile phase was methanol (5%)and ammonium acetate solution (0.02mol/L, 95%) at
a flow rate of 1. 0 ML/min using a UV detector at 214 nm for acesulfame potassiumand 230 nm for benzoic acid and
sorbic acid. The qualitative analysis was madc by retention timc, quantilative analysis was made by cxtcrnal standard
mcthod. The results showcd that the detection limit wasl mg/kg. The methodology exhibited good linearity bctwccn
I 50u/ml with a coefficient of correlation R=0. 95 for acesulfame potassium and R=1. 0000 for benzoic acid and
R=0.99993 for sorbic acid. The average recovery ranged from 89.4 to 94.1 for acesulfame potassium and 97.6%to
101% for benzoic acid and 89.6%to.2% for sorbic acid. The rclativc standard deviation RSD)ranged from 0.38%
收稿凵期:2015-03-10
作者简介:朱小梅(1988),女,助理工程师,主要从事食品安全检测工作。
194
2015年第6期
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