高效液相色谱-串联质谱法测定豆制品中的二甲基黄残留量.pdf

第7卷第7期
食品安全质量检测学报
2016年7月
Journal of Food Safety and Quality
Jul.,2016
高效液相色谱-串联质谐法测定豆制品中的
二甲基黄残留量
丁安帮,熊晓辉”,施祖源,陆利霞2,史听平2,汪开银
(1.国家轻工业食品质量监督检测南京站,南京210002.,南京工业大学食品与轻工学院,南京2100
摘要:目的建立高效液相色谱-串联质谱法( high performance liquid chromatography-tandem mass
pectrometry,HPLC- MS/MS)测定豆制品中的二甲基黄的含量。方法豆制品经乙腈超声提取,经色谱柱
Thermo C18 Acclaim RSLC分离,采用乙腈:水(85:15,K:の作为流动相进行等度洗脱;质谱( electronic spray ion
ESI+)采用多离子监测模式( multiple reactions monitoring.,MRM对二甲基黄的定量离子和定性离子进行监测。
结果本方法在5min内可完成二甲基黄的分离分析。二甲基黄在10-500WgL范围内具有良好的线性关系,在
50、100和200ugL3个添加水平下的加标回收率为90.2%~94.2%,相对标准偏差为0926%(n=9),方法的检出
限为0.4ugkg。结论该方法快速准确、灵敏度高,可适用于测定豆制品中二甲基黄的残留量。
关键词:高效液相色谱串联质谱法;豆制品;二甲基黄
Determination of dimethyl yellow in bean products by high performance
liquid chromatography-tandem mass spectrometry
DING An-bang XIONG Xiao-hui, SHI Zu-hao, LU Li-xia SHI Xin-ping, WANG Kai-yin
(1. The State Light Industry Food Quality Supervision and Detection Station, Nanjing 210009, China
2. College of Food Science and Light Industry, Nanjing University of fechnology, Nanjing 210009, China
ABSTRACT: Objective To develop a method for determination of dimethyl yellow in bean products by high
performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The dried tofu samples
were extracted with acetonitrile by ultrasonic extraction, and separated by Thermo C1& Acclaim RSLC column using
acetonitrile water (85: 15, V: as mobile phase for isocratic elution. Mass spectrometry (ESI+) used multiple
reactions monitoring to identify the quantitative ion and qualitative ion of dimethyl yellow. Results This method
could completc the scparation and analysis of dimethyl ycllow within 5 min. The dimethyl yellow had a good linear
relationship in the range of 10-500 ug/ The recoveries spiked at 3 levels of 50, 100 and 200 ug/L were ranged from
90.2% o 94. 2% with relatve standard deviation(RSD )of 0.926%(m-9), and the limit of detection(LOD)for the
dimethyl yellow was 0. 4 g/kg. Conclusion The proposed method is rapid, accurate and sensitive, which is suitable
or detecting dimethyl yellow in bean products
KEY WORDS: high performance liquid chromatography-tandem mass spectrometry; bean products, dimethyl yellow
通讯作者:熊晓辉,博土,教授,主要研究方向为食品安全。E-mail: cxh(amtech. edu. cn
Corresponding author: XIONG Xiao-hui, P D, Professor, College of Food Science and Light Industry, Nanjing University of Technology,
Nanjing 210009, China. E-mail: xxh@njtechedu cn
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