QuEChERS-超高效液相色谱-串联质谱法快速测定豆制品中二甲基黄和碱性嫩黄O的含量.pdf

第7卷第12期
食品安全质量检测学报
2016年12月
ournal of Food Safcty and Qualily
I)cc..2016
QUECHERS-超高效液相色谱-串联质谱法快速测定
豆制品中二甲基黄和碱性嫩黄O的含量
李锦清,蓁艳,廖桂福,陈满英”
(广东产品质量监督检验研究院,国家食品质量监督检验中心(广东)佛山528300)
摘要:目的建立 QUECHERS结合超高效液相色谱-串联质谱法( QUECHERS- ultra performance liquid
chromatography-tandem mass spectrometry, QUECHERS- UPLC- MS/MS)快速測定豆制品中二甲基黄和碱性嫩黄O
的含量。方法豆制品经20ml』.乙腈-水(1:1,の漫泡,采用 QUF CHERS方法进行萃取、浄化,经Shim-pack
XR-ODS色谱柱分离,正离子扫描.多反应监测模式进行质谱分析,外标法定量。结果二甲基黄在添加水
平为0.5、1和10ugkg时.方法回收率为81.6%97.2%;碱性嫩黄O在添加水平为6、10和50gkg时,方法
回收率为89.19%~1007%:二甲基黄和碱性嫩黄O的相对标准偏差均小于12%6(=6):二甲基黄和破性嫩黄O的
检出限分别为(0.5、2ugkg:定量限分别为1.5、6ugkg。结论该方法操作简便、快速准确、适用于豆制品中
二甲基黄、碱性嫩黄O)的快速筛查和定量分析
关键词: QUF CHERS;超高效液相色谱-串联质谱法;二甲基黄碱性嫩黄O豆制品
Rapid determination of dimethyl yellow and auramine O in bean products b
QUE CHERS method combined with ultra performance liquid
chromatography-tandem mass spectrometry
LI Jin-qing, QI Yan, LIAO Gui-fu, CHEN Man-ying
(Guangdong esting Institute of Produict uality Supervision, China Nation/ uality Supervison and lesting Center
for Foods ( Guangdong), Foshan 528300, China)
ABSTRACT: Objcctive To establish a method for simultaneous determination of dimethyl yellow and auramine O
in bean products by Quechers-ultra performance liquid chromato raphy-tandem mass spectrometry(QUECHERS-
UPLC-MS MS). Methods The bean products were soaked by 20 m acetonitrile-water(1: 1, ]: 7), extracted and
purified by QUECHERS method, separated by Shim-pack XR-ODS III column, and then determined by mass
spectrometry with positive electrospray ionization and multiple reaction monitoring(MRM) mode. xternal standard
method was used for quantification. Results The recoveries of dimethyl yellow were 81.6%0-97.2% when spiked at
0.5, I and 10 ug/kg, and the recoveries of auramine O were 89. 1%-100.7% when spiked at 6, 10 and 50 ug/kg. The
relative standard deviations of the method were less than 12%0(76). The limits of detection of dimethyl yellow and
auramine O were 0.5 and 2 ug kg, respectively. The limits of quantitation were 1.5 and 6 wgk, respectively
Conclusion This method is simple, accurate, and sensitive, which can be used for rapid screening and quantitative
analysis of dimethyl yellow and auramine O in bean products
基金项目:国家质检总局项目(2013QK284)
FAQSIQ(2013QK284
通讯作者:陈满英,博士,高级工程师,主要研究方向为环境检測、食品化工检測。T-mal:-103427809aq.com
esponding author: CHEN Man-ying, Ph.D, Scnior Engi
Edong Testing Institutc of Product Quality
ChinaE-mail:103427809/cqq.com
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