生育酚的薄层色谱快速定性分析.pdf

粮食与食品工业
Vol,24,2017,No.5
Cereal and food Industrv
粮油工程
生育酚的薄层色谱快速定性分析
张翼,郭永红2,胡志雄*,齐玉堂
1.武汉中学(武汉430061)
2.武汉轻工大学食品科学与工程学院(武汉430023)
摘要:建立了一种快速、简单的生育酚单体(g一,Y-和6一生育酚)的薄层色谱定性分析方
法。以硅胶为分离介质,系统考察了由4种溶剂为基础溶剂(环己烷、二氯甲烷、石油醚和氯仿)共
38种溶剂体系为展开剂的分离效果。实验结果表明:当采用100%二氯甲烷、二氯甲烷一乙醚
(98:2.v/v)、二氯甲烷一乙酸乙酯(98:2,v/v)、100%氯仿、氯仿一乙酸乙酯(98:2,v/v)、氯仿一
乙醚(98:2和95:5,v/v)为展开剂时,均能分离3种生育酚单体。其中100%二氯甲烷因具有展
开时间短和操作简单的忧点,成为最适宜建立快速、简单薄层色谱分离的展开剂。在此分离体系条
件下,分离展开时间仅需7min,aー,yー和む一生育酚的比移值分别为0.69.0.55和0.43。
关键词:生育酚;维生素E;薄层色谱:定性分析
中图分类号:TS227文献标识码:A文章编号:1672-5026(2017)05-009-04
Past qualitative analysis of tocopherol with thin-layer chromatography
Zhang Yi, Guo Yonghong, Hu Zhixiong*, Qi Yutang
Wuhan High School (Wuhan 430061)
2. College of Food Science and Engineering. Wuhan Polytechnic University(Wuhan 430023)
Abstract: A fast and simple qualitative analysis of individual tocopherol (a-, y-and 6-to-
copherol)by using thin-layer chromatography(TLC)was established. Silica was used as absor
bant and four types of solvent system(cyclohexane, dichloromethane, petroleum ether and
chloroform used as basic solvents), total 38 solvent systems, were investigated to separate three
tocopherols. Results show that three tocopherols can be separated when using 100 dichlo-
romethane, dichloromethane-ether (98: 2, v/v). dichloromethane-ethyl acetate(98 2,
v/v), 100%chloroform, chloroform-ethyl acetate (98: 2 v/v). chloroform-ether (98: 2 and
95: 5, v/v) as developers. 100% dichloromethane is the optimum developer for establishing a
fast and simple Tl C analytical method owing to having some advantages such as short developing
time and easy operation. Using this TI C method, R, of @-, y-and 6-tocopherol are 0.69
0.55 and 0.43, respectively, and developing time is 7 mir
Key words tocopherol; vitamin E; TLC; qualitative analysis
生育酚是维生素E的重要组成成分,进一步可分为a-,B,Y一和6一生育酚。它具有抗不育、清
收稿日期:2017-08-21
除体内自由基、抗癌、抗心血管疾病、提高免疫力和
作者简介:张翼,男,1999年出生
抗衰老等作用1,近两年的科学研究表明它还具有
汝通讯作者:胡志雄,男,1971年出生,副教授,博士,主要研调节细胞信号和基因表达的非抗氧化作用
究领城为色谱分析
91994-2017chinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp://www.cnki.net