4种黄曲霉毒素B1酶联免疫试剂盒与液相法检测结果比较.pdf

第5卷第3期
食品安全质量检测学报
VOL 5 No. 3
2014年3月
Journal of Food Salcly and Quality
Mar..2014
4种黄曲霉毒素B1酶联免疫试剂盒
与液相法检测结果比较
胡玲玲,项瑜芝,蔡增轩2,任一平2
(1.浙江工业大学化学工程与材料学院、杭州310014:2.浙江省疾病预防控制中心,杭州310051)
摘要:目的综合评价4种不同厂家的黄曲霉毒素B1试剂盒。方法选择几种食品基质(婴幼儿米粉、花
生酱、酱油),分别用R- 13iopharm, Romcr, Iclica和华安麦科的黄曲霉毒素B1试剂盒检测,将测定结果与液相
色谱法測得结果进行比对。结果通过与液相色谱法測得结果比较,华安麦科试剂盒只适合检测婴幼儿米粉样
品。 Iielica和R- Biopharm试剂盒能检测多种基质,而 Telica试剂盒检测结果与液相相符度低, R-biopharm试
剂盒检测米粉时,本底较高。 ROMET试剂盒适用于花生酱和酱油样品。结论为了得到最佳的检测结果,针对
不同的样品基质,应选择合适的试剂盒。当用酶联法检测出阳性样品时,需用液相色谱法确认。
关键词:黄曲霉毒素;酶联免疫法;液相色谱法
A comparative study on 4 kinds of aflatoxin B1 assay kits and
liquid chromatography
HU Ling-ling, XTANG Yu-zhi CAT Zeng-xuan, REN Yi-ping
(1. College of Chemical Fngineering and Materials Science, Zhejiang niversit of techmology, Hangzhou 310014, China
2. Zhejiang Provincia/ Cenlero7disease Conl0/and Prevenlion Langzhou 310051, China)
ABSTRACT: Obiective To compare and evaluate 4 kinds of aflatoxin 13 assay kits and liquid
chromatography Methods Three sample matrixes(infant formula rice flour, peanut butter and soy bean sauce)
were dctccted using RD ASCREINAFLA oxin B, assay, Agraquantaflatoxin B, assay, Itclica low matrix
aflatoxin B assay and Iluaan magncch aflatoxin B assay, and then compared the analysis rcsults with thosc by
liquid chromatography. Results By contrast, Huaan magncch aflatoxin B assay applicd to only the infant
formula rice flour. HELICA low matrix aflatoxin B assay and RIDASCREEN aflatoxin B, assay could dctcct
more sample matrixes, but the analysis results of Helica low matrix aflatoxin B assay were inconsistent with
those by liquid chromatography, the background of RIDASCREENALLATOXIN B, assay was higher
Agraquant aflatoxin B assay were applied to peanut butter and soybean sauce. Conclusion In view of the
dillerent o! the sample matrix, the suilable assay kit needed to be chosen to get the best analysis results. Wher
the results of HI, ISA were positive, the HPI. C method was needed for further validation
KEY WORDS: aflatoxin; enzyme-linked immunosorbent assay; liquid chromatography
通讯作者:任一平,本科,教授级高级工程师,研究方向为卫生理化检验技术及食品安全检测技术。「F-mail:renying(@.263.net
REN Yi-ping Professorate Senior Enginccr.
cial Ccntcr for Discasc Prcvcnti
Bingsheng Road, Binjiang District, Hangzhou 310051, China. E-mail: Icnyiping( 263 nct