黄曲霉毒素B1酶联免疫试剂盒稳定性研究.pdf

※分析检
2013Wo.34,NMo.14315
黄曲霉毒素B1酶联免疫试剂盒稳定性研究
韩春卉,江涛*,李燕俊,高秀芬,李楠,张宏元,赵熙,张靖
(国家食品安全风险评估中心,卫生部食品安全风险评估重点实验室,北京100021)
要:目的:研究自制黄曲莓毒素B(AFB)联免疫试剂盒在不同温度下的稳定性。方法:比较研制出的AFB1醇
联免疫试剂盒在4℃和37℃保存条件下加入不同质量浓度的AFB1后其空白对照孔OD值的变化范围、IC20、ICs、ICa如
及RSD值的变化情况,及标准曲线的R值等指标。结果:4℃保存270空白对照孔OD值变化范围为1.129~1.777
ICa、IC、ICa及RSD分别为0.05ngmL和2736%、0.27 ng/ML和27.58%、0.92ag/mL和19.82%,该保存时间内各标
准曲线线性关系良好(R值均大于0.9):37℃加速破坏条件下保存13d内空白对照孔OD值变化范固为1.200~1.809,
IC2a、ICao、Ca及RSD变化分别为Q.06ng/mL和27,71%、0.25ng/mL和25.66%、1.181 Ing/ML和21.89%,该保存时间内
各标准曲线线性关系良好(R2值均大于0.98)。结论:研制的AFB1醵联免疫试剂盒在4℃和37℃加速破坏条件下分别保
存270d和13d,试剂盒空白对照孔的OD值均大于1.0,各项指标可满足现场检测要求
关词:黄曲審毒素B;醇联免疫试剂盒;稳定性
Stability of Aflatoxin B, ELISA Kit
HAN Chun-hui, JIANG Tao", LI Yan-jun, GAO Xiu-fen, LI Nan, ZHANG Hong-yuan, ZHAO Xi, ZHANG Jing
Key Laboraory of Fo Safety k Asssm Mi of Hal na Naional enter for Food Safety Risk Assessment
Beijing 100021, China)
Abstract: Objective: To xl the effect of rae ma n e stabiliy of he abaory fabricated enzyme linke
immunosorbent assay (ELISA )ki for AF, de ion Method Changes in OD value of blank control wells, Icx, Ics and
I(the concentrations equid for 0, 0% and 80 inh ion, espetivel and RSD were examined upon addition of
different concentration of AFB when ted at 4 r 37 C and the R values of e standard curves al different storage times
were investi. Result On the 270 day of storage a 4 C, the OD value of bank onol wells varied from 1.129 to 1777
and the x IC and IC were 0.05, 0.27 n/m and 0. 2 n/m, with RSD of 27.36%, 27.58% and 19.82%, respectively. The
sanda cues devel d a i e m s u n ae e i d a( er than .9
The OD value of blank control els ared from 1 o 1.80 er a rage pe f 3 ner e aere coins
at 37 C and the Cx Ics and Ia were 0.06, 0.25 n/ML and 1.81 nml with RSD of 27.71%, 25.66%and 21.89
especively.Similarly,thestandardcurvesdevelopedatdiflrentimepointsduringhe270dstorageperiodalsoshowedgood
lin anty(R reater than 0.9 onclusion The OD alue of blank control wells in the ELISA kit remains above 1.0 regardless
of storage at 4 C for 270 d or 7 C for 13 d and all indexes examined can meet the requirements for in situ detection
Key words: aflatoxin B (AFB ) enzyme linked immunosorbent assay(ELISA)kit: stablity
中图分类号;TS201.2
文献标志码:A
文章编号:1002-6630(2013)140315-04
doi:10.7506spkx1002-6630201314066
黄曲毒素B-( aflatoxin B1,AFB1)主要是由曲暮属的究出抵抗黄曲霉侵染和毒素产生的农作物品种。为了减
黄曲霉( Aspergillus flavus)、寄生曲霉( A. paraticus))和少数少人类膳食暴露AFB1,世界上包括我国在内的一百多个
集蜂曲霉(A. nonius)产生的次级代谢产物,对肝脏剧毒
国家和地区制定了食品中AFB1的限量标准,且总的趋势
并有致畸、致癌和致突变作用,被国际癌症研究机构定是越来越严格。我2011年实施的食品安全国家标准食
为类致癌物(人类致癌物)。该毒素广泛污染各类衣产品中真菌毒素限量标准中规定,普通食品中APB1的限量
品、食品及动物倜料,尤以花生及其制品、玉米、大米从5~20 Oug.不等,而婴幼儿配方食品及婴幼儿辅助食
和各类坚果、千果为主。到目前为止,世界各国尚未研品则为0.5ug/kg。由于对粮油食品中黄曲霉毒素的污染
收稿日期:2013-05-06
作者简介:韩春卉(1964-),女,副主任技师,学士,研究方向为食品卫生。E-mail:hanchunhuit028@163.com
通信作者:江涛(1971ー),男,研究员,硕士,研究方向为食品卫生。E-mail:jiangtaol025@163.com
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