凝胶渗透色谱-气相色谱法测定猪肉中11种有机磷农药残留.pdf

第5卷第6期
食品安全质量检测学报
VOL 5 No 6
2014年6月
Journal of Food Salcly and Quality
um..2014
凝胶渗透色谱-气相色谱法测定猪肉中
11种有机磷农药残留
高雪',王翠芬,刘萍,刘晓慧,齐雅薇,颜红,孔春梅2,刘小宝2
(1.保定出入境检验检疫局,保定071051;2.保定职业技术学院,保定071051)
摘要:目的建立凝胶色谱技术(GPC)净化,气相色谱法測定猪肉中11种有机磷农药残留量的分析方
法。方法样品中的待测农药组分经丙酮提取,GPC净化去除油脂等杂质,经旋蒸、氫吹、浓缩后,进入GC
分析,外标法定量。结果有机磷在002~1gmL浓度范围内具有良好的线性响应,相关系数在0.999以上,加
标量在0.01mgkg时,加标回收率在83.4%~-105.6%之间,相对标准偏差在2.15%~6.52%之间,检出低限为001
mgkg。结论该方法简便、快速、分离度好,、适于批量样品的测定。
关键词:猪肉;凝胶色谱;气相色谱:有机磷;农药残留
Determination of 11 organophosphorus pesticide multi-residues in pork b
gas chromatography with gel permeation chromatography
GAO Xuc WANG Cui-fcn Llu Ping LIU Xiao-hui, QI Ya-wci', YAN Hong
KONG Chun-mei2 LIU Xiao-bao?
(1. Baoding Enry-exit Inspeclion and Ouaramline Bueau, Baoding 071051, China
2. Baoding Vocational and Technical College, Baoding071051, China)
ABSTRACT: Objcctive To develop a method for determination of I I organophosphorus pesticide residues
in pork by gas chromatography(GC)with gel permeation chromatography(GPC). Methods The samples o
pork were extracted by acetone, cleaned up by GPC to remove grease and other impurities. Alter spin steaming,
nitrogen blowing, enrichment, the analyte was determined by (C using the external standard method. Results
The method showed a good linearity over the rangc of 0.02-1 u/mt for II organophosphorus pesticide resi
dues, with correlation coefficients above 0.999. The recoveries were 83.4%-105.6% at the spiked level of
0.01 mg/kg, with relative standard deviation between 2.15%0-6.52%. Ihe low detection limit was 0.01 mg/kg
Conclusion The method is simple, rapid, and has a good separating degree, and it is suitable for the batch
sample detcrmination
KEY WORDS: pork; gel chromatography; gas chromalography organophosphorus; pesticide residues
基金项目:河北出入境检验检疫局自主立项项目(HE2012K003)
Fund: Supported by the Hebei Entiy-exit Inspection and Quarantinc Burcau Independent Projcct (HE2012K003) utonomy Projccts
(l112012K003
通讯作者:刘晓慧,博士,主要研究方向为动物检疫。T-mail:disha163.com
Corresponding author: I Xiao-hui, ID, Baoding Entry-eit Inspcction and uarantine Burcau, Baoding 071051, China. E-mail
drlxh(a163.com