高效液相色谱法同时测定食品中纳他霉素和富马酸二甲酯的含量.pdf

第8卷第6期
食品安全质量检测学报
Vol 8 No 6
2017年6月
Journal of Food Safety and Quality
2017
高效液相色谱法同时测定食品中纳他霉素和
富马酸二甲酯的含量
官咏仪,郵志超2”,金梦,宋F旧,张美金,陈毓芳,谢玉珊
(1.广东出入境检验检疫局检验检疫技术中心,广州510623;2.广州质量监督检測研究院,广州511447)
摘耍:目的建立高效液相色谱法同时测定食品中纳他霉素、富马酸二甲酯的分析方法。方法样品用甲
醇溶解提取、超纯水定容, Agilent TC-C1s柱色谱分离,采用高效液相色谱-光电二极管阵列检测器进行检测,
并以峰面积外标法定量。结果在4种基质、4个添加水平下,纳他霉素的平均回收率在88.19%~97.7%之间,相
对标准偏差为2.47%-5.72%,检出限为0.10mgkg;富马酸二甲酯的平均回收率在84.5%~104%之间,相对标
准偏差为1.21%~5.28%,检出限为0.05mgkg。结论该方法具有操作简便、快速、准确的特点,适用于食品
中纳他霉素、富马酸二甲酯的含量分析。
关键词:高效液相色谱法;纳他霉素;富马酸二甲酯
Simultaneous determination of natamycin and dimethyl fumarate in foods by
hig
gh performance liquid chromatography
GUAN Yong-yi QIU Zhi-chao", JIN Meng, SONG Yang, ZHANG Mei-jin
CHEN Yu-fang XIE Yu-shan
(1. inspection and Quarantine echnology Center, Guangdong Entry-exit Inspecton and Quarantine Bureau
Guangzhou 510623, China: 2. Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China)
ABSTRACT: Obiective To establish a method for simultaneous determination of natamycin and dimethyl fumarate
(DMF)in foods by high performance liquid chromatography (HPLC). Methods Samples were extracted by
methanol and water, separated by Agilent Tc-cis column, detected by HPLC with photo-diode array detector(PDA
and quantified by the external standard method. Results At 4 different spiked levels in 4 kinds of food, the average
recoveries of natamycin were between 88.1%-97.7%, with the RSD of 2.47%-5.72%. The average recoveries of
dimethyl fumarate were between 84. 5%-104%, with the RSD of 1.21%5.28%. Detection limits of natamycin and
DMF were 0. 10 mg/kg and 0.05 mg/kg, respectively. Conclusion This method is simple, fast and accurate, which
can be applied to the analysis of the content of natamycin and DMF in foods
KEY WORDS: high performance liquid chromatography; natamycin; dimethyl fumarate
松素,是一种重要的多烯类抗菌素,能够有效抑制酵母菌
等真菌的生长,且其难溶于水和油脂,难以被人体消化、
纳他霉素( natamycin),也称游链霉素、匹马菌素或海
吸收,大部分摄人的纳他霉素会随粪便排出,对人体造成
*通讯作者:邱志超,硕士,质量工程师,主要研究方向为食品质量与安全检测。E-mai:392479231goq.com
Corresponding author: Q Zhi-chao, Master, Quality Engineer, Guangzhou Quality Supervision and Testing Institute, No 1-2, Zhujiang Road
ChaoTianIndustrialZone,ShilouTown,PanyuDistrict,Guangzhou511447,ChinaE-mail:392479231(@qq.com
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