大孔树脂吸附纯化番木瓜籽总三萜研究.pdf

第6卷第6期
食品安全质量检测学报
Vol. 6 No. 6
2015年6月
Journal of Food Salcly and Quality
um..2015
大孔树脂吸附纯化番木瓜将总三萜研究
李幼梅,周楷,白新鹏,张云竹
海南大学食品学院,海口570228)
摘要:目的筛选出适宜吸附纯化番木瓜籽总三萜的树脂类型,初步确定其吸附-解析工艺条件。方法采用
S-8、NAK-9、AB-8、HPD700和D-1015种不同极性大孔树脂对番木瓜将总三萜进行吸附纯化,通过比较5
种树脂对番木瓜籽总三萜的吸附解析性能及产品回收率,筛选出最佳树脂类型及确定吸附纯化工艺。结果
试验表明,相较其他4种树脂,弱极性树脂AB-8较适用于番木瓜将总三茜的吸附纯化、建立了AB-8静态吸
附总三萜的动力学方程,确立了AB-8纯化总三茜的工艺条件,对树脂AB-8的等温吸附进行初步探讨。结论
在洗脱剂乙醇体积分数为80%、吸附2h、解析2h.AB-8大孔树脂对番木瓜将总三萜纯化效果较佳,产品的解
析率达70.89%,回收率达到59.58%0
关键词:番木瓜将;总三茜;大孔树脂;纯化
Study on purification of total triterpenes from papaya seeds with
macroreticular resins
LI You-mci, ZHOU Kai, BAT Xin-pcng, ZHANG Yun-zhu
(College of Food Science and Fngineering, Hainan (niversity, Haikou 570228,(hina)
ABSTRACT: jective To filtrate the best macroreticular resins for purification of total triterpenes in papaya
seeds and to confirm the technological paramctcrs. Methods The purification of total triterpenes from papaya
seeds with 5 types of macroporous resins S-8, NAK-9, AB-8, HPD700, and D-101 was studied. The optimal resin
and parameters for purification were confirmed by comparing the properties of adsorption and desorption and
recovery. Results Compared to other resins, AB-8 was the most appropriate resin for the purification of papaya
seeds total triterpenes. The static adsorption kinetics equation of total Triterpenes of AB-8 had been established
Conclusion Validation experiments indicated that with ethanol desorption agent concentration of 80, 2 h
adsorption and desorption 2 h, desorption ratio and product purity could reach 70.89% and 59.58%, Respectively
KEY WORDS: papaya seeds; tolal triterpenes; macroporous resin purification
瓜等,属十字花目番木瓜科水果,原产于中美洲,现
广泛分布于世界各国。据FAO统计,全球番木瓜年
番木瓜( Carica Papaya L-),名万寿果、番瓜、木产量超1000万吨,我国超50万吨,在广东、海南、
基金项目:2014年海南省研究生创新科研课题(Hys2014-27)、海南大学研究生优秀学位论文培育计划第一批资助
Iund: Supported by 2014 Postgraduate Student Innovation Scientific Research Project of I linan Province(1lys2014-27) the Iirst Lundin
Projecl o[ Fxeellenl Disserlation OI Postgraduate of Hainan Universily
通讯作者:张云竹,副教授,主要研究方向生物资源利用。 li-mail: langue23G163.c0m
*Corresponding author: ZLIANG Yun-zhu, Associate Professor College of lood Science and Inineerin, Ilainan University, llaikou 570228,
China.E-mail:lamsuc123(@163.como