水酶法提取红花籽油工艺的研究.pdf

食品与发酵科技
Food and Fermentation Technolog)
第52卷(第1期)Wn.52,An.1
水酶法提取红花籽油工艺的研究
李倩,赵丽,马媛,朱丹实,王勃,惠丽娟,何余堂,马涛,刘贺
渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,
生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013
摘要:以红花将为原料,在胰珻的最适pI条件下,采用中心组合设计对胰酶用量及料液比、解温虔和时间进行优
化,确定水酶法提取红花籽油最佳工艺为:酶添加量为305ug,料液比16,提取温度45℃,提取时间为3h,红花籽油的
最大提取率约为65.14%:气相色谱测定结果表明:水响法得到的油脂由11种脂肪酸组成,其总含量高达90%以上,其
中饱和脂肪酸以棕榈酸为主,不饱和脂肪酸以亚油酸和油酸为主,其中亚油酸含量最多,相对含量约为79%
关鍵词:红花将;水酶法;油得率;响应面分析;气相色谱
中图分类号:T201.1;TS2.55.19
文献标识码:A
文章编号:1674-.506X(2016)01-0053-0008
Study on Extraction Process of Safflower Oil by Aqueous Enzymatic
LI Qian, ZHAO Li, MA Yuan, ZHU Dan-shi, HUI Li-juan, WANG Bo, HE Yu-tang,
MA Ta0. IU He
(1.College Q/Food Science and echnology, ohai niuersiy, Jin hou Lioning 121073, hin:
2. Food Safety Key Lab of Liaoning Prouince, inghou Liconing 121013, China
3. tiona 1 cal Joint Engineering Reserch enter of torg oce sing and afet Control Technology for Fresh
gricultural and Aquntic Proucts, Jinzhou Liaoning 121073, hin
Abstract Central Composite e ign was applied lo optimize the aqucous cnymalie exi ration paramelers of safflow
cr seed oil. The optimal hydrolysis condition were malerial liquid ralio of 1 6, Trypsinase amount of 305u/% and
temperature ol 45, hydrolysis time of 3h as the discovery through response surface methodology. According to the
optimal parameters, lhe validation extraclion experiments have been carried oul, and the maximal exiraction rale of
oil was 65.I4%, the resu was close to Ihe predicted value. By using GC analysis, II ally acids were detected in
the safflower oil, including saturated atty acids (mainly palmitic acid)and unsaturated fally acids (mainly oleie acid
and linoleic acid). Among these fatty acids, the most abundant was linoleic acid with a relative content of 799
Key words safflower seed; aqueous enzymatic; oil yield; response surface methodology; GC
doi:10.3969/issn.1674-506X.2016.01-011
红花( Carthamus tinctorius L.)又名草红花、菊用,其将含油一般在23-46%,高于大豆。红花将油
红花等,是一年生双子叶菊科草本植物,集油、药、中必需脂肪酸含量达80%以上,其中亚油酸的含量
染料、饲料兼用,其花冠、将实、茎叶、積秆都可以利高达73-85%,是已知植物油中亚油酸含量最高的。
收稿日期:2015-11-06
基金项目:国家自然科学基金面上项目(31471621);国家自然科学基金青年基金项目(31201385);辽宁省高等学校优秀人才支
持计划(IR2014034):锦州市科学技术计划项目(12AIB27)
作者简介;李倩(1991-),女,在读硕士研究方向:粮食、油脂及植物蛋白工程
通讯作者:刘贺(1979-),男,博士,教授。研究方向:食品大分子的结构与功能及其修饰
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