植物油中壬基酚检测方法的探讨.pdf

Vol.23,2016,No.1
行食与食品工业
Cereal and Food Industry
标准与检测
植物油中王基酚检测方法的探讨
郭宗敏,刘红玉,孙淑华
山东西王食品有限公司(邹平256209)
摘要:王基酚是一种环境污染物,具有雌激素样效应,能够对机体产生毒害作用。据调查,王
基酚普遍存在各类食品中,能从包装材料或通过其他途径向食品中迁移,如塑料制品中的壬基酚向
植物油中迁移等。但目前,国内尚未有植物油中壬基酚的国家或行业检測标准,相关文献资料也甚
少。本文根据油脂特性,摸索了植物油中王基酚的检测方法:称取一定质量植物油加入无水乙醇超
声提取30min,并离心取上清液、过有机澽膜,利用高效液相色谱荧光检测器(发射波长为227nm
激发波长为315m)进行检測。本文所述方法检出限为10pgkg,回收率可达80%以上。
关键词壬基酚;植物油高效液相色谱冫荧光检测器
中图分类号:IS227文献标识码A文章编号:1672-5026(2016)01-084-03
Exploration on determination of nonylphenol in vegetable oil
Guo Zongmin, Liu Hongyu, Sun Shuhua
Shandong Xiwang Foodstuffs Co., Ild. (Zouping 256209)
Abstract Nonylphenol is a kind of environmental pollutant, having an estrogen-like effect, and
can produce toxin effect on the body. According to the survey, nonylphenol generally exists in all kinds of
foods. It can migrate to food from packaging materials, for example, migrating lo vegelable oil from plas
tics. At present, there is no national standard or industry standard on determination of nonylphenol in
vegetable oil, and there is a paucity of literature in domestic. According to the characteristics of vegetable
oil, the determination of nonylphenol in vegetable oil was explored. Firstly, the vegetable oil samples
having certain quality and added to anhydrous alcohol were extracted by ultrasonic. Secondly, the vegeta
ble oil samples were detected by HPL C equipped with a fluorescence detector(the emission wavelength
227 nm and the excitation wavelength is 315 nm) after cen ion. Detection limit of the method is 10
pb and the rate of recovery of the method can reach more than 80%o in this paper
Key words nonylphenol, vegetable oil, HPLC, fluorescence detector
壬基酚( nonylphenol,NP)不是自然界天然存在工业中的应用也非常广泛,可用于生产酚醛树脂、可
的物质,是酚类烷基化过程中形成的工业合成物,主塑性聚酯、抗氧化剂及聚氧乙烯稳定剂等,在食品包
要用于生产非离子表面活性剂(王基酚聚氧乙烯装材料、容器内壁涂料等方面有着非常重要的用途。
醚,NPEO)。同时,王基酚作为塑化剂在食品包装王基酚具有难降解特性及疏水性、脂溶性和生物累
积性,属于环境雌激素物质。一旦进入生物体内后,
它就会影响生物体正常的生殖和发育,可以导致人
作者简介:郭宗敏,1983年出生,中级工程师,高级质量工程类男性精子数量的减少。目前,王基酚主要是作为
收稿日期:2015-07-14
师,主要从事液相色谱、质谱检测工作
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