人参药材HPLC指纹图谱和高端保健品多维指纹图谱的研究.pdf

河北大学
硕士学位论文
人参药材HPLC指纹图谱和高端保健品多维指纹图谱的研究
姓名:苑若瑶
申请学位级别:硕士
专业:分析化学
旨导教师:闫正
20090601
摘要
摘要
本学位论文共分为四章
第一章:文献综述。主要概括了中药指纹图谱的定义和分类,总结了中药指纹图谱
在中药质量控制上的重要作用,以及其发展现状和研究进展。讨论了研究中药指纹图谱
的技术路线及中药指纹图谱质量评价体系的建立。
第二章:人参药材 HPLC指纹图谱的研究。建立了人参的高效液相指纹图谱,研究了
不同的预处理方法、流动相组成、最佳检测波长、色谱柱温度等操作因素对人参指纹谱
图的影响,并对不同产地和种类的人参药材的指纹图谱进行了比较。色谱条件为:不锈
钢填充柱( Inertsil ODS-3,250mmX4.6mm,5m)色谱柱;检测波长为203mm;流动
相为乙腈-0.05%磷酸水溶液梯度洗脱;流速:1mL/min;分析时间:90min;柱温为30
℃。该实验方法所建立的人参药材的HPLC的指纹图谱的分析周期较短,预处理方法简
单,可为人参药材质量控制提供参考,并可为其它中药材指纹图谱的建立提供借鉴。
第三章:鹿茸药材HPCE指纹图谱的研究。建立了鹿茸的毛细管电泳指纹图谱。研
究了不同的预处理方法、缓冲溶液及添加剂、最佳检测波长、电泳电压、电泳温度等操
作因素对人参指纹谱图的影响,并对不同产地的鹿茸药材的指纹图谱进行了比较。电泳
条件为:未涂渍弹性石英毛细管柱(75m×60cm):缓冲溶液为60 mmol/L-40 mmo/L
尿素硼砂混合溶液;检测波长为200m;电泳电压为20kV;进样时间为3s;进样压
力为14kPa;电泳温度为室温。该实验方法所建立的鹿茸的HPCE的指纹图谱预处理方法
简单,分离度较好,可为区别原产地与非原产地高端保健品鹿茸提供参考依据。
第四章:冬虫夏草HPLC指纹图谱研究探索。对不同产地种类的冬虫夏草进行高效
液相指纹图谱的比较研究。采用HPLC-UV指纹图谱分析条件:Cs色谱柱;流动相为:乙
腈和0.05%磷酸水溶液的梯度洗脱,检测波长203nm,运行时间90分钟,进样体积为20
uL,柱温为30℃。实验表明,采用HPLC的方法,操作方便,分析速度快,分离效率高,
检测灵敏度高,进样量少,为达到指纹图谱测定的要求提供了方便,通过对分离条件的
优化,峰型和出峰时间都有了比较好的改善。
关键词高端保健品人参鹿茸冬虫夏草 PIPLC HIPCE多维指纹图谱
Abstract
Abstrac
This paper consists of four chapters.
In chapter one, we literature and review the definition and classification of the
fingerprint chromatogram of Chinese traditional medicine and its important roles in quality
control and development. At the end of the paper, we talk aboat the technology lines on
fingerprint chromatogram of Chinese traditional medicine research and discuss how to
establish the quality evaluation eystem of it
In chapter two, an analytical method of fingerprint of Ginseng by HPLC was established
We studied different sample pretreatment, detection wavelength, mobile phase, temperature
and compared the fingerprint chromatograms of Ginseng from six different areas and
kinds Column Inertsil ODS-3(250 mm x 4.6 mm, 5um, )was used with the mixture of
acetonitrile-0.05% phosphoric acid; the detection wavelength was 203 nm; the flow rate was 1
ml/min and the column temperature was 30 C
In chapter three, an analytical method of fingerprint of Com Cervi Pantotri chum by
HPCE was established. We studied different sample pretreatment, CE buffer and additive
detection wavelength, run voltage and compared the fingerprint chromatograms of Corn
Cervi Pantotrichum from six different areas. A 75 u m x 60cm uncoated capillary was used
with the mixture of 60mmo1/l-40mmoll urea-borate acid as CE buffer the detection
wavelength was 200 nm; the run voltage was 20KV the inject time was 30s the sampling
pressure was 14kpa and room temperature.
In the last chapter, we research on how to establish the fingerprint of Cordyceps sinensis
by HPLC,. We studied different sample pretreatment, detection wavelength, mobile phase,
temperature and compared the fingerprint chromatograms of Cordyceps sinensis from three
different areas. Column Inertsil ODS-3(250 mm 4.6 mm, 5 um, )was used with the mixture
of acetonitrile-0.05% phosphoric acid; the detection wavelength was 203 nm; the flow rate
was I ml/min and the columm temperature was 30 C
Key words Senior Healthy Products Ginseng Corn Cervi Pantotri chum
Cordyceps sinensis HPLC HPCE multidimensional Fingerprint