二甲亚砜提取——冷冻离心净化测定食品中苯并【a】芘及苯并【e】芘.pdf

烺油食品科技第25卷2017年第2期
质量安全
DOI:10.16210j.cnki.1007-7561.2017.02.014
甲亚砜提取一冷冻离心脊化测定
食品中苯并[a]及苯并[e]花
邓丽娟2,洪霞,钱滢文2,高志莹2
何海宁2,刘琦2,周鑫魁'2
(1.甘肃省商业科技研究所,甘肃兰州730010
2.甘肃中商食品质量检验检测有限公司,甘肃兰州730010)
摘要:建立以二甲基亚砜作为提取溶剂,冷冻离心提取净化食品中的苯并[a]芘和苯并[e]茈,高
效液相色谱一荧光法检测的方法。替代传统的固相萃取小柱净化方式,能更快速、高效、准确检测
食品中苯并[a]茈和苯并[e」茈的含量。2种目标物在0.5~1000ng/mL范固内呈现良好线性关
系,在1~100ng/g添加量下,回收率为89.0%~91.3%,相对标准偏差为1.19%~1.29%;仪器检
出限为0.01ng/mL,植物油脂方法检出限为0.05n/g,肉制品和动物油脂方法检出限为0.02ng/g
该方法测定动植物油脂和肉制品中的苯并[a]茈和苯并[e]茈方便、快捷,适用于批量化检测。
关键词:二甲亚砜;苯并[n]芘和苯并[e]茈;动植物油脂;冷冻离心
中图分类号:TS201.6文献标识码;A文章编号:1007-7561(2017)02-0069-05
Determination of benzo a pyrene and benzo e pyrene in food by
extraction with dimethyl sulfoxide and purification with refrigerated centrifugation
DENG Li-juan", HONG Xia"",QIAN Ying-wen
GAO Zhi-ying", HE Hai-ning", IIU Qi", ZHOU Xin-kui
(1. Gansu Business Science and Technology Institute, Ianzhou Gansu 730010
/hongshang Food Quality Test and Detection Co .1d..Ianzhou G
Abstract: Benzo [I a] pyrene and benzo [] pyrene in food was extracted and purified by refrigerated
centrifugation with dimethyl sulfoxide as solvent and analyzed by Hplc-fluorescence detection method
This method can replace the traditional solid-phase extraction column purification method, which is fas
ter, more efficient and accurate in the detection of benzo a] pyrene and benzo [e ] pyrene con
Both target object showed a good linear relationship within range of 0.5-1000 ng/ML, when addition
was 1 100 ng/g, the recovery rate was 89.. 3%, and the relative standard deviation was
1. 19%0-1.29%; the detection limit was 0. 01 ng/ml, vegetable fat detection limit was 0.05 ng/g,
meat and animal fat detection limit was 0.02 ng/g. This method is faster and more convenient in the de
tection of benzo [ a] pyrene and benzo [e] pyrene in animal, vegetable fat and meat products, which is
suitable for batch detection
Key words: dimethyl sulfoxide; benzo [a pyrene, benzo [e! pyrene; animal and vegetable oils and
fats; refrigerated centrifugation
苯并[a]芘(BaP)结构为含有五个苯坏的稠坏芳香烃。是一种高活性致癌剂,是世界公认的
大強致癌物质(黄山莓毒索、苯[a]芘、亚硝胺)之
收稿日期:2016-08-24
12-3。小剂量苯并[a]芘就能引起局部组织
基金项目:中国-马来西亚科技交流合作项日(1604WKCA001);
啉-青海食品研究开发与检测联合实验宰建设经费项目
变,也可引起大鼠外周血淋巴细胞DNA、肺细胞以
606RTSA37)
作者简介:邓阿,1988年出生,女,倾士,工程师
及肝细胞损伤+?。食品在加工过程中很容易广生
通讯作者:周鑫魁,1986年出4,男,硕上,工程师.
苯并芘,例如汕料在晾昞过程中可能受到并芘污
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