二甲亚砜促进5-氨基酮戊酸光动力疗法对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应.pdf

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倪文琼,等二甲亚砜促进5一氨基酮戊酸光动力疗法对A431细胞杀伤和小鼠皮肤鳟癌的抑制效应
甲亚砜促进5-氨基酮戌酸光动力疗法对A431细胞杀伤和小鼠皮
肤鳞癌的抑制效应
倪文琼,张晨阳,季艳芳,肖春才,张雪,李存涛
Enhanced 5 -Aminolevulinic acid-based photodynamic effects by Dimethyl sulfoxide
on A431 cytotoxicity in vitro and inhibition of skin squamous carcinoma in mice
Ni Wenqiong, Zhang Chenyang, Ji Yanfang, Xiao Chuncai, Zha
untao
Department of Dermatology, Zhengzhou Central Hospital, Zhengshou Uninersity, Henan Zhengzhou 450007, China
Abstract Objective: To study the effect of enhanced 5-aminolevulinic acid-based photodynamic therapy
(ALA-PDT) by DMSO on A431 cytotoxicity in vitro and inhibition of mice skin squamous cell carcinoma( MSCC)in
vivo. Methods: A431 cells were co-cul ured with DMSO Apoptosis after ALA-PDT and protoporphyrin X(Pp)
concentration of A431 cells co-cul ue wh DMSO were detected by flow cytometry. The fluorescence intensity in
MSCC frozen sections was detected by laser scanning confocal microscopy. After ALA-PDT the MSCC suppress
rates were compared. Results: The Pp IX fluorescence intensity of 431 cells in the experimenta therapeutic group
howed DMSO dose dependant manner, was higher than that in the ALA alone or control group(P<0.01 ) After
ALA-PDT, the A431 cell apoptotic rates in the experimental roup were higher than that in the ALA alone or eontrol
group(P <0.01). The Ppx fluorescence intensity in the experimental group showed DMSO dose dependant manner
was higher than that in the AL A alone or control group (P <0. 01). After ALA-PDT the tumor suppressing rates
showed DMSO dose dependant manner in therapeutic group(P<0.05) Conclusion DMSO can promote ALA-PDI
effects on A431 cytotoxicity and MSCC inhibition
( Key words DMSO; 5-aminolevulinic acid; photodynamic therapy; apoptosis; tumor suppressing rates
Modern Oncology2013,21(12):2674-2677
摘要】目的:研究DMSO参与5-氨基酮戊酸介导的光动力疗法(ALA- based photodynamic therapy,ALA
PDT)对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应,探讨治疗皮肤肿瘤的优化作用。方法:DMS0千预后,采
用流式细胞仪检测A431细胞内PpX水平和PDT后细胞凋亡率。激光共聚焦显微镜检测小鼠鳞寤瘤体组织
的荧光强度,比较PDT后各组鳞癌小鼠的抑瘤率。结果:实验组A431细胞PpⅨ荧光强度率高于ALA组和空
白对照组(P<0.01),与DMSO含量呈浓度依赖性。PDT后实验组细胞凋亡率高于ALA组和空白对照组(P
<0.01)。实验组瘤体组织PpⅨ荧光强度值高于10%ALA对照组和10%DMSO对照组(P<0.01),与DMS0
含量呈浓度依赖性。实验组鱗癌小鼠PDT治疗后,抑瘤率高于对照组(P<0.05),并有量效依从关系。结
论:DMSO可促进ALA-PDT对A4311细胞杀伤和小鼠皮肤鳞癌的抑制效应。
【关键词)】二甲亚砜;5-氨基酮成酸;光动力疗法;细胞凋亡;抑瘤率
中图分类号】R730.5;R739.5【文献标识码】ADO1:10.3969/j.isn.1672-4992.2013.12.09
文章编号】1672-4992-(2013)12-2674-0
随着光动力疗法( photodynamic therapy,PDT)在皮肤肿挑战性的课题。国内外应用5-氨基嗣戊酸(5- Aminolev
瘤治疗领域中的广泛应用,相关疗效调控的研究就成为极富 ulinic acid,ALA)介导的光动力疔法治疗非黑色素性皮肤肿
一直受到关注。作为影响疗效因素之一的光敏剂,具有的
水溶性特性,对皮肤或细胞膜的渗透性及弥散浓度有限,限
【收稿日期】2013-06
制了ALA-PDT对于深度大于10mm的皮肤肿瘤的治疗。
修回日期】2013-06-27
本研究通过促进渗透剂二甲亚砜(DMSO)对体外培养A431
【基金项目】郑州市普通科技攻关项日(编号:112 PPIISF312-
【作者单位】郑州大学附属郑州中心医院皮肤科,河南郑州
和小鼠鳞癌模型的光动力效应的影响,为探讨DMSO干预后
ALA-PDT治疗皮肤肿瘤的优化作用提供依据。
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【作者简介】倪文琼(1962-),女,上海人,主任医师,从事皮肤性
树料与方法
病专业,主要从事皮肤病理,皮肤肿瘤研究。E-mei,1.1主要试剂和伩器
656014316@ gc. corn
人表皮细胞癌细胞株A431(南京凯基生物公司);二甲