天然水溶性甲壳素溶液的性质.pdf

2012年12月
食品研究与开
第33卷第12期
研究
38
Food Research And Development
天然水溶性甲壳素溶液的性质
刘廷国“2,李斌
(1.华中农业大学食品科技学院,湖北武汉4300702.池州学院材料与化学工程系,安徽池州247100)
摘嬃:利用冻融循环处理结合均相脱乙酰制备了天然水溶性甲売素(WSC)。在3种不同溶剂中,紫外光语差异不
大。脱乙酰度一定时,水溶性甲壳素样品的水溶液在208m~212nm的吸光度A与波长入星线性关系,其斜率与対
应溶液的淑度呈线性关系,初步建立了1种用紫外多波长回归测定WSC水溶性的方法。圆二色语显示,在稀酸溶液
中水溶性样品比脱乙酰度相当的非水落性样品分子統更加仲展。冻融術环和均相脱乙誌打破了分子间强氢相互
作用,使WSC的空间结枸更为舒展,較少呈现螺旋结构,更易溶于水中
关键词:水溶性甲壳素;冻融循环;紫外光语;圆二色谱
the Solution Property of a Natural Water-soluble Chitin
Liu'ting-guo'2, LI Bin
(1. Colege of Food Science and Technology, Huahong Agricultural University, Wuhan430070, Hubei
China: 2. Department of Material and Chemical Engineering, Chizhou University, Chizhou 24710, Hubei
Abstract: A natural water-soluble chitin(WS)was prepared by alkaline treatment under homogeneous
conditions using freeing thawing cyclic treatment. There was no ifference between the ultra voile
spectroscopy in three different solvents. In aqueous water-soluble chitin solution, there was a linear relationship
between absorbance(A and wavelength()in the range of 208 nm-212 nm, when the degree of deacetylation
was as constant. And there was also a good linear relationship between the value of the slope and the
concentration of WSC. As a result, a multiwavelength linear re ression ultra voilet spectrophotometry was
established for determining water solubility of WSC. The circular dichroism spectra showed that in diluted acid
solution. there was more extend molecular chain of WSC than half deacetylated non-water-soluble chitin. It
meaned that freezing -thawing -cylic treatment and homogeneous deacetylate broke the strong moleeular
hydrogen bonds, made WSC have more stretching spatial structure, less helix structure, and led it soluble in
waler more cally
Key words water-soluble chitin; freezing-thawing-eylic treatment; ultra voilet spectroseopy circular dichro-
壳聚糖( chitosan)是甲壳素(chin)的部分N-脱乙现的唯一带正电荷的天然碱性多糖,具有多种特殊生
酰产物。甲壳素在自然界中的生物合成量仅次于纤维物活性,安全无毒,对环境无公害。广泛应用于农业、
素,估计每年自然界生物合成量将近10"是迄今发工业、畜牧业、环境保护、日用化T.、化学分析以及食
品、医药卫生、生物工程等领域。
基金項目:安徽高等学校省级自然科学研究项目(KI201B110);池
由于分子内、分子间存在强氢键相互作用,甲壳素
学院化学材料与工程省级示范实验中心建设项目(統教高〔208)和壳聚糖均不溶于普通溶剂,限制了其广泛应用。通
4号);池州学院材料学院级重点学科建设项目(2011XK04,2012CL001)
过均相脱乙酰化或均相乙蹴化控制脱乙酰度在50%
作者简介:刘廷国(1982-),男(汉),讲师,士,研究方向:大然高
分子改性与应用
左右制备的半脱乙酰甲壳素能溶于水中?,但对其性
*通信作者:李斌(1972-),男(回),博士,教授,博上生导师。
质研究不够深入,目前仅限于实验室少量制备,尚不
万方数据