基于HepG2细胞模型研究普洱茶茶色素的抗氧化作用.pdf

2262017,Vo.38,No.01
※营养卫生
基于HepG2细胞模型研究普洱茶茶色素的
抗氧化作用
陈亚蓝,王雪青*,王恰雯,郑子晴,叶美霞,付芳芳,宋文军,王素英,白晓丽,李长文
(1.天津商业大学生物技术与食品科学学院,天津市食品与生物技术重点实验室,天津300134
2.云南天士力帝泊洱生物茶集团有限公司,云南普洱65100)
摘要:采用正常培养和油酸诱导培养的HepG2细胞为模型,通过测定普洱茶茶色素对HepG2细胞外谷胱甘肽
( glutathione,GSH)含量,过氧化氢酶( catalase,CAT)活力和细胞内丙二醛( malondialdehyde,MDA)含量的
影响,以研究普洱茶茶色素的抗氧化作用。结果显示,普洱茶茶色素对正常培养的HepG2缃胞的抗氧化作用影响不
显著,而对油酸诱导的HepG2细胞模型抗氧化作用有显著的提高。抗氧化作用提高的程度依赖于普洱茶茶色素的质
量浓度。当400ugmL普洱茶茶色素作用HepG2油酸诱导模型24h,细胞外GSH含量由0044g几L增加到0.0103gL,
CAT活力由0.136U/mL提高至1.174U/mL,细胞内MDA含量由15.146 nol/mg减少到7.63nmol/mg,从而使这些指
标接近正常培养HepG2细胞模型水平。因此,普洱茶茶色素的抗氧化作用是通过提高清除活性氧的酶活力和促进合
成还原性物质来干预细胞的氧化应激。
关健词:HepG2:抗氧化;细胞模型;普洱茶;茶色素
Antioxidative Capacity of Pigments from Pu-erh Tea Based on HEPG2 Cell Model
CHEN Yalan WANG Xueqing*, WANG Yiwen ZHENG Ziqing, YE Meixia, FU Fangfang
SONG Wenjun, WANG Suying BAI Xiaoli, LI Changwen
( l Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,
Tianjin 300134, China; 2. Yunnan Tasly Deepure Biological Tea Group Co Ltd, Pu Erh 665100, China)
Abstract: In terms of catalase(CAT)activity and malondialdehyde(MDA )and glutathione(GSH) levels, the antioxidative
capacity of pigments from Pu-erh tea was assessed using Hepg2 cell models cultured with normal or oleic acid enriched
medium. The results showed that the antioxidative capacity of Pu-erh tea pigments was significant on the oleic acid-induced
HEPG2 cell model in a concentration-dependent manner rather than on the normally cultured ones. The extracellular GSH
content and CAT activity in HEPG2 cells induced by oleic acid for 24 h with added Pu-erh tea pigments increased from 0.0044
to 0.0103 g/ and from 0.136 to 1. 174 U/ml, respectively, and MDA content reduced from 15. 146 to 7.635 nmol/mg, almost
reaching the normal values. Therefore, the antioxidative mechanism of Pu-erh tea pigments may be due to enhancing reactive
oxygen species(ROS)scavenging enzyme activities, promoting the s nthesis of reducing substances and therefore altering
the status of oxidative stress of HEPG2 cells
Key words: HEPG2; antioxidant capacity; cell model, Pu-erh tea; tea pigments
DOH:10.7506/spk~x1002-6630-201701038
中图分类号:R151.4
文献标志码:A
文章编号:1002-6630(2017)01-0226-06
引文格式
陈亚蓝,王雪青,王佁雯,等.基于HepG2细胞模型研究普洱茶茶色素的抗氧化作用门.食品科学,2017,38(1):226-231
DOI:10.7506/spkx1002-6630-201701038.http://www.spkx.net.cn
CHEN Yalan, WANG Xueqing, WANG Yiwen, et al. Antioxidative capacity of pigments from Pu-erh tea based on Hepg2
cell model [J]. Food Science, 2017, 38(1): 226-231. (in Chinese with English abstract )DO: 10.7506/spkx1002-6630-
201701038.http://www.spkx.net.cn
收稿日期:2016-03-14
基金项目:天津市高等学校创新团队建设规划资助项目(TD12-5049):天津市高校科技发展基金资助项目(20120603);
天津商业大学大学生创新训练项目
作者简介:陈亚蓝(1991-),女,硕士研究生,主要从事天然活性物质的研究与开发。E-mail:704930060@q-com
勇偏傅袭王雪青(1ー)?女、教授?博土?主要从事天然活性物质的研究与开发。E-mail:wxqing@ticu.edu.cn