脱脂棉固定化脂肪酶PCL催化合成乙酸癸酯.pdf

验研究
中国食品添加剂
hina Food Additives
脱脂棉固定化脂肪酶PCL催化合成乙酸癸酯
张莹,周美娟,丛方地,2,王晓红
张树林2,刘海学3,任健,杨薇
(1.天津农学院基础科学学院生物制药系,天津300384;2.天津农学院天津市水产生态及养殖
重点实验室,天津300384;3.天津农学院农业分析中心,天津300384)
摘要:乙酸癸酯是一种广泛用于食品和医药品的芳香添加剂。因天然提取量少,不能满足需求,通常
利用合成方法制备乙酸癸酯。由于化学法污染环境严重、产率低且杂质多,而非水相酶催化合成乙酸癸脂是
种绿色合成途径,而且副反应少、产率高、产物易于分离提纯。所以,选用假单胞菌脂肪酶( Pseudomonas
cepacia lipase,PCL)为生物催化剂,在37℃和160rmin的条件下,通过物理吸附,将PCL固定在脱脂棉上,
制备脱脂棉固定化PCL,并用于催化正癸醇与乙酸乙烯酯反应,合成乙酸癸酯。结果发现,酶与棉的质量比为
10:12时,脱脂棉固定化PCL的活性最高,在37℃和160 r/min,催化反应6h,转化率达95%以上。在同
样条件下,PCL酶粉仅能催化29%的底物反应。如果重复利用这种固定化酶,每次催化6h,第3次的转化率
仍可达到92%。在25で和静置条件下,6h后,固定化酶依然能转化底物89%。可见,脱脂棉固定化酶PCL
的催化活性明显提高,且其有低碳催化特性。
关键词:乙酸癸酯;固定化脂肪酶;酶活性;酶稳定性
中图分类号:TQ925+.6/1Q656+,1文献标识码:A文章编号:1006-2513(2017)12-0051-06
Synthesis of decyl aceate catalyzed by cotton immobilized lipase PCL
ZHANG Ying ZHOU Mei-juan, CONG Fang-di. 2", WANG Xiao-hong
ZHANG Shu-lin? LIU Hai-xue3. REN Jiant. YANG Weil
(1. Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University
Tianjin 300384; 2. Tianjin Key Lab of Aquatic Ecology and Aquaculture, Tianjin 300384
3. Agricultural Analysis Center, Tianjin 300384)
Abstract Decyl acetate is an aromatic additive widely used in food and medicine. Due to the lack of natural extraction
amount, the demand cannot be met and thus usually prepared by synthesis methods. The chemical synthesis method
has the problem of serious pollution, low yield with many impurities, however, on contrary, the enzymatic
reaction in non-aqueous phases is a green synthesis method, and has advantages such as less side reaction, high
yield, and the product is easy to separate and purify. Thus, Pseudomonas cepacia lipase(PCL), as a biocatalys
was immobilized on absorbent cotton by physical adsorption at 37C and 160 r/min. The prepared cotton-immobilized
PCL(cotton PCL )was used in catalyzing reaction of decyl alcohol to vinyl acetate. The results showed that, cotton
PCL displayed better catalytic activity when the mass ratio of enzyme to cotton was 10: 12. After catalysis for 6 h at
收稿日期:2017-07-16
通讯作者
基金项目:天津市国家级大学生创新创业训练计划项目(20141006136,201710061012):天津市水产生态及养殖重点实验室开放基
金项目(TJAE201502):国家自然科学基金项日(3110442)
作者简介:张莹(1995-),女,山西临汾人,本科生,从事非水相酶催化方面的研究,E-mai:lm13022280730163com
2017年第12
万方数据