固相萃取-HPLC法测定辣椒油中偶氮染料.pdf

2017年7月
食品研究与开发
标准与检测
140一
第38卷第13期
Food Research And Development
DOI:10.3969/jsn.1005-6521.2017.13.029
固相萃取-HPLC法测定辣椒油中偶氮染料
邢燕,高慧,刘玉栋,王勤
(1.淄博市疾病预防控制中心,山东淄博255026;2.淄博市科学技术情报研究所,山东淄博255025
摘要:建立一种基于大孔吸附树脂柱对辣椒油中苏丹红I-IV、苏丹红7B和对位红等6种偶氮染料进行的前处理
方法。辣椒油样品经正己烷稀释,用自制大孔吸附树脂柱净化,用高效液相色谱一二极管阵列检测器检測,自制固相
萃取柱经再生过程可重复使用。结果显示,6种染料在005g/nl.~10g/mnl.浓度范国内线性关系良好,相关系数高
于0998,在3个加标浓度下,日内和日间的加标回收率在90.9%~104.3%之间,相对标准偏差(RSD)小于3.7%。
关键词:偶氩染料;辣椒油;固相萃取;液相色谱;大孔吸附树脂
Determination of Azo Dyes in Chilli Oi by SPE Coupled with HPLC
XING Yan, GAO Hui, LIU Yu-dong, WANG Qin
(1. Zibo Municipal Center for Disease Conlrol and Prevention, Zibo 255026, Shandong, China; 2. Science and
Technology Information Research Institute, Zibo 255025, Shandong. China)
Abstract: An innovative pretreatment method for 6 kinds of azo dyes, such as Sudan I-IV, Sudan 7B, Para
red, in chilli oil based on homemade macroporous resin solid-phase extraction(SPE )cartridge was established
The chilli oil samples were diluted by n-hexane and purified by a self-made macroporous resin cartridge, de
termined by high performance liquid chromatography (HPLC )coupled with diode array detector (DAD). The
homemade SPE cartridge simplified the preparation procedure of samples and could be reused by regeneration
steps. Results showed that the recoveries of 6 azo dyes added to the chilli products had a good linear in the rang
rom 0.05 Mg/Ml-1.0 wg/ML. The correlation coefficients were more than 0.999 8 for all experimental batches
Under three spiking levels, the recovery rates of intra-day and inter-day were ranged from.9% to 104.3
and the relative standard deviations(RSDS )were lower than 3.7 0
Key words: azo dyes chilli oil; solid-phase extraction; HPLC; macroporous resin
基金项日:山东省自然科学基金项日(R2015PH035);山东省医药卫生科技发展计刘项日(2013WS0028):浙江省科技厅分析测试项日
(2015C37009)
作者简介:邢燕(1982-),女(汉),主管技师,博士,从半食品、环境中有机污染物检测研究
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收稿日期:2017-01-11
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