高效液相色谱-质谱联用检测水产品中氧化三甲胺.pdf

第7卷第1期
食品安全质量检测学报
Vol. 7 No l
2016年1月
Joumal of Food Safety and Quality
高效液相色谱一质谐联用检测水产品中氧化三甲胺
付雪媛,刘丰海2,姜城子,薛长湖,王玉明
(1.中回海洋大学食品科学与工程学院,青岛2660032.青岛市市立医院检验科,青岛266071)
摘要:目的建立水产品中氧化三甲胺( trimethylamine-N- oxidc,TMAO)的高效液相色谱法-质谱联用
(HPLC-MS/MS)分析方法,并对2种常见水产品的TMA0含量进行測定。方法待测物通过色谱柱分离,以10
moVL甲酸铵为流动相A,含5 mmol/L甲酸铵、25%甲醇、0.1%甲酸溶液为流动相B进行悌度洗脱,流速为
0.8mL/min,采用多反应监测(MRM)检测不同撞击电压下母离子碎裂情况,并选择合适电压下的离子对定量
利用所建立方法测定阿根廷鱿鱼和凡纳宾对虾中TMAO含量。结果当撞击电压均设定为20eV,确定定量离
子对为mk76→58.2时,5~1000gL范用内线性关系良好;4种水产品TMAO回收率在83.15%~90.239%之间。
结论所建HPIC-MSMS测定TMAO的方法,特异性好、灵敏度高,快速准确,可用于水产品中TMAO含量
的分析测定。
关鍵词:氧化三甲胺;高效液相色谱-质谱联用法;水产品
Determination of trimethylamine-n-oxide in aquatic product using high
performance liquid chromatography-tandem mass spectrometry
FU Xue-yuan LIU Feng-hai', JIANG Cheng-zi', XUE Chang-hu", WANG Yu-ming
(1. College of Food Science and Engineering, Ocean University of China, ingdao 266003, China
2. Qingdao Municipal Hospital Inspection Depariment, Oingdao 266071, China)
ABSTRACT: Objective To develop a method for measurement of trimethylamine-n-oxide(TMAO)in
aquatic products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and
detect the TMAO content in 2 kinds of familiar aquatic products. Methods The analytes were separated on a
chromatographic column at a flow rate of 0.8 ml/min using 10 mmol/L ammonium as mobile phase A and the
solution contained 5 mmol/L ammonium formate, 25% methanol, 0.1% formic as mobile phase B
Multip e-reaction moni orin (MM was used to determine the transition of precursor ion to product ion at a
eries collision emergy and one ion pair at the appropriate collision energy was selected to quantification. The
TMAO content in / ex argentinuscastellanos and Litopenaeus vannamei was determined using the developed
method. Results The method had a good linear relationship ranged 5-1000 ug/L when the collision energy was
set to 20 ev and m/z 76-58.2 was selected as quantitative ion pair as well. The recovery rates of 4 kinds of
aquatic products were 8315%09023. Conclusion The established HPLC-MS/MS method for TMAO showed
good specificity, sensitivity, and simplicity, which was applicable for monitoring TMAO in aquatic products
基金项目:国家自然科学基金项目(31371757)、新世纪优秀人オ支持计划NCET-3-0534)
Fund: Supported by the National Natural Scicncc Foundation of China (31371757)& Program for New Century Excellent Talents in University
(NCET-13-0534)
通讯作者:王玉明,博士,教授,主要研究方向为水产食品营养学。E-mal:wangyuming@ouc.edu.cn
Corresponding author; WANG Yu-ming, PHD, Professor, College of Food Science and engineering, Ocean University of China, Qingdao
266003,China.E-mail:wangyuming(@ouc.edu.cn
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