香草醛-硫酸测定甘草浸膏中甘草酸含量.pdf

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化工进展
180
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2013年第32卷第1期
研究开发
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香草醛-硫酸测定甘草浸膏中甘草酸含量
张慧慧,刘明言,凌宁生
(天津大学化工学院,天津30002:2天津中新药业集团股份有限公司中新制药厂,天津300457)
摘要:为了建立甘草提取甘草多糖后浸膏中甘草酸合量的测定方法,以香草醛-硫酸为显色剂,535nm为最大
吸收波长,进行了比色条件优化及方法学考察。结果表明:在0.25mL待测液中,加入0.25mL浓度为6mgml
的香草醛溶液和2.0mL75%碗酸,在温度为75℃条件下反应25min,显色效果最好。该法精密度高(RSD-4.93%,
n=6),1h内稳定(RSD=1.09%,=60),平均加样回收率为95.93%(RSD=4.62%,n=5),是一种简便、准确、
稳定和重复性好的检测脱糖甘草漫膏中甘草酸含量的方法。
关键词:甘草浸膏;甘草酸;香草醛-硫酸;含量测定
中图分类号:TQ461
文献标志码:A
文章编号:1000~-6613(2013)01-0180-05
DOI:10.3969 ].issn.1000-6613.2013.01.030
Determination of glycyrrhizic acid in licorice extractum by
colorimetry
ZLANG Haihui, LIU Mingyan LING Ningsheng
( School of Chemical Engineering, Tianjin University, Tianjin 300072, China; Zhongxin Pharmaceutical Factory,
Zhongxin Pharmaceuticals Group, Ltd, Tianjin 300457, China)
Abstract: In order to establish a method of determining the content of glycyrrhizic acid in licorice
extractum after polysaccharide was extracted, the optimization of chromogenic conditions and
investigation of methodology were carried out with vanillin-sulfuric acid as color development reagent
and 535 nm as the maximum absorption wavelength. Optimal reactive conditions for 0.25ml sample
solution were obtained as follows: color development reagents 2.5 ml 6 mg/ml vanillin and 2.0 ml
75% H2SO], temperature 75 C, reaction time 25 min. This method had high precision(RSD=4.39%,
n=6), stability (RSD=1.09%, n =60 )and average recovery(y-95.93%, RSD=4.62%, n 5) and was
reliable to determine the glycyrrhizic acid in licorice extractum after polysaccharide was extracted
featuring convenience, accuracy, high stability and reproducibilit
Key words: licorice extractum; glycyrrhizic acid; vanillin-h2soa; content determination
甘草为豆科( Leguminosae)甘草属取完甘草多糖后的甘草浸膏中提取甘草酸的研究
( Glycyrrhiza)植物,主要有效成分为三萜化合物、报道
黄酮类和多糖化合物等?。甘草酸是甘草的根和
目前,甘草酸含量测定方法主要有:重量法、
根茎中所含的一种五环三萜皂苷,具有抗炎、抗氧比色法、紫外分光光度法、薄层扫描法、高效液
化、抻菌等作用叫,但过量服用甘草酸可能引起钠相色谱法及毛细管电泳法的等。高效液相色谱和
潴留和高血压。甘草中含有多类有效成分,甘草
提取物多是利用甘草酸。为了节约资源,需对提取收稿日期:201207-20:修改稿日期:2012-08-0
甘草酸后的残余成分开展利用研究。大多研究为对
基金项目:国家“十一五”科技计划项目(2009ZX09301-008-P-11)。
第一作者:张慧慧(1985一),女,硕土。联系人:刘明言,博士,教
提取完甘草酸后甘草渣的利用,但是尚未见到从提投。E-mailmyliu@tju.edu.cn