黄腐酸对单针藻Monoraphidium sp.FXY-10油脂积累及me、pepc、accD基因表达量的影响.pdf

108
CHINA OILS AND FATS
2018Vol.43No.7
生物工程
黄腐酸对单针藻 Monoraphidiumn sp.FXY-10油脂积累
及me、pepc、acD基因表达量的影响
董训赞,车绕琼,赵永腾,李涛,徐军伟,赵鹏,余旭亚
(昆明理工大学生命科学与技术学院,昆明650500)
摘要∷硏究了黄腐酸对单针藻 Monoraphidium sp.FXY-10生长、油脂含量、活性氧(ROS)水平及与
油脂合成相关基因表达的影响。采用两阶段培养方法(第一阶段异养培养,第二阶段自养培养),
并添加黄腐酸诱导。结果表明:在25mg/L黄腐酸诱导下,6d时藻细胞中油脂含量可达54.3%
是对照组的1.12倍,藻细胞内活性氧水平是对照组的1.46倍。此外,油脂合成相关酶基因与油脂
合成存在相关性,黄腐酸诱导下,me、acD、pepc基因表达量分别是对照组的1.32倍(4d)、1.67倍
(4d)及1.1倍(6d)。研究表明,黄腐酸提高油脂积累可能与活性氧水平及基因表达有关。
关键词: Monoraphidium sp.FXY-10;黄腐酸油脂ROS;基因表达
中图分类号:TK6:Q81
文献标识码:A
文章编号:1003-7969(2018)07-0108-05
Effects of fulvic acid on lipid accumulation and genes(me, pepc, accd
expression of Monoraphidium sp. FXY-10
DONG Xunzan, CHE Raoqiong, ZHAO Yongteng, LI Tao
XU Junwei, ZHAO Peng, YU Xuya
(Faculty of Iife Science and Technology, Kunming University of Science and Technology, Kunming 65050. China
Abstract The effects of fulvic acid on the biomass, lipid content, reactive oxygen(ROS)level and the
expression of lipid biosynthesis-related genes of Monoraphidium sp. FXY-10 were studied. Two stage
culture method (first heterotrophic and then photoautotrophic)was used and fulvic acid was added to in-
duce. The results showed that the lipid content reached 54. 3% in algal cells treated with 25 mg/L fulvic
acid, which was 1. 12 times of the control, and the intracellular ROS level was 1. 46 times of the contro
Moreover, the lipid biosynthesis-related genes was correlated with the lipid synthesis, and the expres-
sions of me, aced) and pepe were 1.32 times(4 d), 1. 67 times(4 d)and 1. 71 times(6 d)of the con
trol. These results demonstrated that fulvic acid significantly increased microalgae lipid accumulation b
changing the ROS level and gene expression
Key words: onoraphidium sp. FXY-10, fulvic acid, lipid, ROS, gene expression
人类利用能源的方式在继木柴向媟炭、煤炭向是一种清洁的可再生能源,是化石能源的良好替代
油气的转换已基本完成之后,将经历油气向新能源品。利用微藻生产生物柴油具有诸多优势,因
的第三次重大转換。化石燃料的过度开发和使此受到越来越多国内外学者的关注。
用,造成了严重的能源危机和环境问题。生物柴油
添加外源诱导剂可以影响微藻的生长和油脂的
积累。Zhao等的研究表明,外源添加5mmol/L
收稿日期:2017-09-29:修回日期:2018-04-24
基金项目:国家自然科学基金项目(21766012,21666012)
的甜菜碱可以显著提高微藻中油脂含量。而10
作者简介:置训赞(99),男,在读硕士,研究方向为生物质mg/L黄腐酸同样可作为植物诱导剂促进 Haemato
能源(R-mi)xunzandonm_knust@163.com
coccus pluvialis的生长和虾青素的积累。黄腐酸
通信作者:余旭亚,教授( E-uuail) xuya YL163.co
是有机肥的重要组成部分,含有丰富的营养元素,能
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