农药三唑酮分子印迹聚合物的识别特性研究.pdf

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第26卷第4期
河地大学学报(自然科学版)
2006年7月
ournal of Hebei University(Natural science Edition
Jul.2006
农药三.唑酮分子印迹聚合物的识别特性研究
吴文镶',李莉2,杨更亮4,李志伟,张国庆4
廊坊师范学院继续教育中心,河北廊坊0650002.河北理工大学轻工学院,河北唐山063020;
(3.中国科学院化学研究所分子科学研究中心,北京100080;4.河北大学化学与环境科学学院,河北保定071002)
摘要:采用分子印迹技术合成了对农药三唑酮有高选择性的印聚合物,通过 Scatchard分桁研究了
印迹燹合物的結构特性.结果表明,以甲基丙烯酸为功能单体的印迹聚合物,通过氬鍵作用可以形成两类结
合位点.用多点结合模型计算两美结合位点的离解常数分刚为K1=7.89×104molL,K2=3.53
10~3mol/L.底物的选择性结合实验表明,该聚合物三唑酮呈现高度的选择性及识别能力.为在生物样品
中选择富集三唑酮提供了可能性
关键词:分子印迹技术;印迹聚合物;二唑酮;底物选择性
中图分类号:O652.6
文献标识码:A
文章编号:1000-1565(2006)04-0396-05
Characteristics of Molecular Recognition of Triadimefon Imprinting Polymer
WU Wer-xiang, LI Ii", YANG Geng-liang", LI Zhi-wei, ZHANG Guo-qing
(1. Center of Continued Education, Langfang Teachers College, Langfang 065000, China
Tangshan 063020, Ch
3. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
4. College of Chemistry and Environmental
Hebei University, Baoding 071002, Ch
Abstract: A molecular imprinting polymer with high selectivity for triadimefon was prepared by a molecular
imprinting technique. The selective binding characteristics of the imprinting polymer was evaluated by Scatchard
analysis. The multiple-sites binding model was used to calculate the maximum number of binding sites and the
dissciation constant. The results showed that the imprinting polymer using methacrylic acid(MAA) as func
tional monomer could form two kinds of binding sites. The dissociation constants were estimated to be K
7.89 mo/L and K23. 10 mol/L. The selective binding experiment for substrates indicated that
the polymer gave much higher affinity and selectivity for triadimefon than for triadimenol and imidacloprid. It
s possible to be a good way in selective enrichment and determination of trace Triadimefon in complex samples
Key words: molecular imprinting technique; imprinting polymer; triadimefon; substrate selectivity
分子印迹技术是指为获得在空间结构和结合位点上与某一分子(印迹分子)完全匹配的聚合物的实验制
收稿日期:2005-06-25
基金项目;廊坊师范学院自然科学资助项く ISZYZ200401
作者简介:吴文镶(1964-),女,河北文安人,廊坊师范学院副教授,从事有机化学教学工作