流动注射光度法测定植物油中的脂肪醛.pdf

第8卷第2期
食品安全质量检测学报
2017年2月
Journal of Food Safety and Quality
Fcb.、2017
流动注射光度法测定植物油中的脂肪醛
龙泽荣,刘莉,赵建勇,鹿榖",王利涛2,赵亮2,董树清,李辉2
(1.新疆维吾尔自治区产品质量监督检验研究院、乌鲁木齐83000
2.中国科学院兰州化学物理研究所,兰州730000
摘要:目的建立流动注射法检測植物油中醛类化合物的方法。方法利用醛类物质和间苯三酚在酸催化
作用下生成红色单甲川类衍生物的原理,在自制的流动注射分析仪中,以相同摩尔量的甲醛、乙醛、正丙醛、
正丁醛、正戌醛、正己醛、正庚醛、正辛醛和正王醛配成的混合醛为标准物质,通过优化反应中的酸催化
剂种类及用量、温度、试剂浓度以及流速等参数,确定最佳的流动注射法检測植物油中的醛类物质方法。
结果最佳的流动注射条件为:两个蠕动泵的流速控制在O.5mL/min,检测波长为455mm,进样量为100pL
恒温水浴槽的温度为75℃。在该条件下,醛类化合物在(0.00003mlL浓度范围内具有良好的线性关系
线性方程为Y=3.19×105X+1.12×105,=0.98:;该方法的相对标准偏差为0.419%~2.19%,回收率在90%以上。
结论该方法可用于实际食用油样品中醛类物质的检测,具有方法简单、测试快速、数据准确的特点,可在
食品检测领域中获得推广。
关键词:流动注射分析;分光光度法;植物油;醛类化合物
Determination of aldehydes in vegetable oils by flow injection
spectrophotometry
LONG Ze-rong, LIU Li, ZHAO Jian-yong, LU Yi", WANG Li-tao, ZHAO Liang"
DONG Shu-qing, LI Hui-
1. Xinjiang Uygur Autonomous Region Produc/uality Supervision and Inspection Institute, Urumqi 830001
China: 2. Lanzhou Institute o/Chemical Physics of the Chinese Academy ofsciences, Lanzhou 730000, China
ABSTRACT: Objective To establish a method for the determination of aldehydes content in vegetable oils with flow
injection spectrophotometry. Methods The standard substance was made up of mixed aldehydes with the same mole
of formaldehyde, acetaldehyde, n-propanal, n-butanal, n-pentanal, n-hexaldehyde, n-heptaldehyde, n-oclanal and
n-nonanal. The best determination condition of aldehydes was determined by the optimization of acid catalyst
tcmpcrature, conccntration and flow ratc paramctcrs using the rcaction of aldehydes and phloroglucinol to produc
the red methenyl derivatives undcr the acid action by homc-made Mlow injcction cquipmcnt. Results The optimal
flow injection conditions for the experiment were as follow: determination wavelength was 455 nm, the flow rates of
two peristaltic pumps were 0.5 ml/min, the injection volume was 100 HL, and the temperature of thermostatic water
bath was 75 C. In optimal conditions, the Favourable lincar rclation of aldehydes was in range o!0.0006-003 moll
基金项目:国家自然科学基金项目(21405162,21405161)、乌鲁木齐市科技局重点项目(NY141320007)
Fund: Supported by the National Natural Science Foundation of China(21405162, 21405 161)and Key Projects of Urumqi City Technology
Bureau(Y141320007)
通讯作者:鹿毅,提高待遇高级工程师,博士生导师,主要研究方向为食品分析和精细化学。F-mail:luyi_ xixi(a163.con
*Corresponding author: I Yi, Professor, Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Urumqi
830001,ChinaF-mail:luyixjxjy(@163.com
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