液相色谱法检测番茄汁中杀菌剂和杀虫剂的研究.pdf

食品与酵科技
Food and Fermentation Technolog
第50卷(第6期)Wol.50,No.6
液相色谱法检测番茄汁中杀菌剂和杀虫剂的研究
史龙梅,李欢,周建科
(河北大学理化分析中心,河北省分析科学技术重点实验室,河北保定071002)
摘要:采用分散液液微萃取方法提取番茄汁中三种杀蔺剂(三唑酶、灭荫丹、五氧硝基苯)和四种苯甲脲杀虫剂(除
虫脲、杀铃脲、氟钤脲、氟苯脲)两类农药,高效液相色谱法对提取组分进行测定。最终确定了以25suI.二氧甲烷作卒
取剂和1.0mI.甲窮作分散剂最佳萃取条件;色谱条件为:反相C18色谱柱分离,甲醇:乙購;水=50:25:25(w/,甲酸调
H=3.7)为流动相,蒙外检测波长225nm;七组分在所测范目内线性关系良好,相关系数大于0.992,方法检出限在
0.2-2.0ng/mI.之间,相对标准偏差小于3.4%。
关键词:高效液相色谱;分散液液微萃取;番茄汁;杀荫剂;苯甲酰脲杀虫剂
中图分类号:TS207.7
文献标识码:A
文章编号:1674-506X(2014)06-0070-0004
Determination of Fungicides and Insecticides in Tomato Juice
by Lliquid Chromatography
Sill Long-mei. LI lluan. ZILOU Jian-ke
Research Center of Physics and Chemistry Analysis, Key L boratory analytical Science andd Technology of
Hebei Prorince, Hebei Universily, Boodling 071002, hina)
Abstract Employing dispersive liquid-liquid microextraction(D. MP)method to extract the three kinds
fungicides and four kinds of benzoylurea pesticides in tomato juice. Triadimenol, Folpet, PCNB, Diflubenzuron
Triflumuron, Hexaflumuron, Telubenzuron were separaled by reversed-phase High Performance liqui
chromatography. Finally we identified the Optimum extraction conditions (255WL of Dichlormethane as extracting
agent and 1.0ml of methanol as dispersant )A Diamonsil C18 (5um, 250mmx4 6mm i,d) was used for separation
of the target compounds. The mobile phase was the mixture of methanol, acetonitrile and water (50: 25: 25, V/, the
oli was adjusted by formic acid to 3.7) and detection wavelength was 225nm. The linearity of regression equations
were good in the range of measuring amount, with correlation coefficients were greater than 0.9992, Limit of
detection is 0.2-2.0ng/ML, The relative standard deviations(RSD) were less than 3.4%
Key words: HPLC; DLLME; Tomato juice; Fungicides; benzoylurea insecticides
doi:10.3969/j.is.1674-506X.2014.06-016
环境介质及食品中农药残留的危害已不言自灭菌丹、五氯硝基苯较为常用,特别是番茄生长过程
明,众所周知。水果蔬菜中若有农药残留将直接威胁中病害的防治,其线留危害甚至可能出现致癌、致
到粜蔬汁饮料的质量和安全。杀菌剂是用于作物生畸、致突变的情况1国家标准2规定番茄中五氯硝
长后期或采收后病害防治的一?类农药,其中三唑醇、基苯最人残留限量≤0.1mgkg
收稿日期:2014-08-26
作者简介:史龙梅(1988-),女,硕士在读研究生。研究方向:色谱分离与分析。
通讯作者:周建科(1956-)男,硕十土研究生导师。研究方向:色谱分离与分析。
21994-2015ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp://www.cnki.net