基于肝微粒体和肝S9成分的食品接触材料中五氯苯酚体外代谢分析.pdf

第7卷第1期
食品安全质量检测学报
Vol 7 No
2016年1月
Journal of Food Safety and Quality
Jan.。2016
基于肝微粒体和肝S9成分的食品接触材料中
五氯苯酚体外代谢分析
龚强,丁利,朱绍华,成婧,焦艳娜,付善良,王利兵
湖南出入境检验检疫局检验检疫技术中心,国家食品安全检测重点实验室,食品安全科学技术溯南省重点实验室
长沙410004
摘要:目的通过体外代谢方法,研究五氯苯酚( pentachlorophenol,PCP)的代谢情况。方法采用肝微粒体
和肝S9成分2种体外代谢試剂模拟体内代谢模式,通过优化代谢反应条件,建立PCP、四氯苯(CI4BQ)2种
目标物的气相色谱-质谱联用(GCMS)检测方法,对PCP进行体外代谢研究分析。结果体外代谢分析的最佳
反应条件为代谢试剂浓度0.75 mg]L、代谢反应时间4h。结论在最优反应条件下分析比较2种试剂对PCP
的代谢情况,验证了P(P的代谢产物为Cl4BQ.且在肝微粒体和肝S9成分中的代谢没有显著差异。
关键词:五氯苯酚;四氯苯醌;肝微粒体;肝S9成分;气相色谱-质谱联用法;体外代谢
Metabolic study of pentachlorophenol in food contact materials by liver
microsome and liver $9 fraction
GONG Qiang, DING Li, ZHU Shao-hua, CHENG Jing, JIAO Yan-na, FU Shan-liang, WANG Li-bing
( Technology Center of Funan Enmy-exit Inspection and uarantine Bureaw, State Key Laboratory of Food Safery Testing,
Hunan Key Laboratory of Food Safety Science Technology, Changsha 410004, China)
ABSTRACT: Objective To analyze the metabolite of pentachlorophenol (PCP) through simulating
metabolism in vivo. Methods Liver microsomes and liver S% fraction were used as two kinds of in vitro
metabolism reagents to simulate in vivo metabolic pattem. The method for the determination of PCP and
tetrachloro-1, 4-benzoquinone( was established by gas chromatography-mass spectrometry(gc-ms)
through optimizing the metabolic reaction conditions, and then in vitro metabolism of PCP was analyzed
Results The best metabolic reagent concentration was 0.75 mgml, and the most suitable reaction time was 4
h. Conclusion The two rcagcnts of ICP metabolism under the optimal reaction conditions were compared
Cl4 BQ was validated to be the metabolite of PCP, and there was no significant difference between the
metabolist composition of liver microsomes and liver S9 fraction
KEY WORDS: pentachlorophenol; tetrachloro-1,4-benzoquinone; liver microsome; liver S9 fraction; gas
chromatography-mass spectrometry; metabolism in vitro
基金项目:国家科技支撑计划项月(2012BAD29B05
Fund: Supported by the Natiunal Key Technology Research and Development Program of the Ministry of Science and Technology of China
(2012BAD29B05)
通讯作者:工利兵,博士,研究员,主要研究方向为食品安全与检验检疫安全。E-meil: wanglu049@c126.om
corresponding author: WANG Li-bing, Ph D Researcher, Hunan Key Laboratory of Food Safety Science& Technology, Technology Center
ofHunanEntry-exitInspectionandQuarantineBureau,Changsha410004,ChinaE-mail:wanglb419(@126.com
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