AuNCs无标记癌胚抗原免疫传感器的研究.pdf

第26卷第5期
化学研究与应用
2014年5月
Chemical Research and Application
Mav,2014
文章编号:1004-1656(2014)05-0652-06
基于HGB/PT/ AUNCS无标记癌胚抗
原免疫传感的研究
赵锦航,牛小方,郑丽,赵锦芝,葛艳珊,杨云慧
(云南师范大学化学化工学院,云南昆明650092)
摘要:本文研制了一种基于中空石墨烯球(HGB)/聚硫莖/金纳米笼的无标记型癌胚抗原传感器。将合成的
中空石墨烯球修饰于玻碳电极(CCE)表面,在其上电聚合硫堇形成多孔聚硫堇(PTh)复合膜后吸附固定金纳
米笼,然后将癌胚抗体(ani-CEA)固定到电极表面,制得无标记型癌胚抗原(CEA)免疫传感器。当抗体与抗
原发生免疫反应时,形成的复合物阻碍了电子传递,从而降低聚硫堇的电催化活性。通过示差脉冲伏安法
(DPV)检测聚硫堇的响应电流信号的降低,实现对CEA的无标记检测。在最优的实验条件下,检癌胚抗原
的线性范围为0.5~80gmL',线性相关系数为0.992,检测限为0.17ng-mL'。该免疫传感器可用于临床
上CEA的检测。在血清样品中进行了CEA的检测,结果令人满意
关键词:中空石墨烯球;癌胚抗原;聚硫莖(PIh);金纳米笼( AUNCS);免疫传感器
中图分类号:0657
文献标志码:A
A label-free immunosensor for the determination of carcinoembryonic
antigen based on HGB/PTH/AUNCS
ZHAO Jin hang, N]U Xiao-fang, ZHENG Ii, ZHAO Jin-zhi, GE Yan-shan, YANG Yun-hui
( College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China
Abstract: In this paper, a label-free immunosensor for carcinoembryonic antigen was developed based on hollow graphene ball/pol
thionine/gold nanocage. Synthesized hollow graphene ball(HGB )was modified on the surface of glassy carbon eleetrode(GC ) ol
lowed by e]ectropolymerizing thionine on it to adsorb gold nanocage Carcinoembryonic antibody(an. A)was immobilized on the
surface of electrode to construct label-free immunosensor for the determination of carcinoembryonic antigen(CA). When ANTI-CEA
binds with CEA antigen, the formed immunocomplex obstructed electron trans er, which decrease the electrocatalysis of PTH. The de
crease of differential pulse voltammetry(DPV )response of PTH was used to realize label-free detection of carcinoembryonic anti-
gen. Under the optimal experimental conditions, the linear range was 0.5-80 ng ml with the detection limit of 0. 17 ngml
the linear correlation coefficient was 0.9932. This immunosensor has been used to detect CEA ir serum sample, the results were sat
Key words: hollow graphene ball(HGB); carcinoembryonic antigen(CEA); Polythionine( Pth);gold nanocage(AUNCS ); immu-
sensor
癌胚抗原(CEA)是国际公认的肿瘤标志物,于1965年被发现,是一种具有人类胚胎抗原特性
它是癌细胞膜上一种极为复杂的含糖结构蛋白,决定簇的酸性糖蛋白,存在于成人癌组织中,是
收稿日期:201309-27;修回日期:2014-08-18
基金项目:国家自然科学基金项目(21165023)资助
联系人简介:杨云慧(1963-),女,教授,博土,主要从事电化学及生物传感器的研究。E-mail:yhui2002 o aliyun,com