离子交换树脂对白扦中莽草酸的分离纯化.pdf

化工进展
2009年第28卷第1期
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
145
研究开发
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离子交换树脂对白扦中莽草酸的分离纯化
杨磊,刘玉,张琳,祖元刚
(东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040)
摘要:建立了离子交换树脂分离纯化白打中莽草酸的工艺条件和参数。通过研究D261、D296、D301-R、D301-G、
D290、201*7(717)、D201和D280共8种离子交換树脂对莽草酸的吸附和解吸附能力,筛选出最佳树脂为D290,确定
了最佳的吸附与解吸附工艺参数,吸附条件为pH=6、25℃、流速为3mL/min;脱附条件为:洗脱液为2.5%NaOH水
溶液,洗脱流速为3 mlmin。莽草酸样品溶液经D290树脂吸附与脱附后回收率为92.53%,纯度由2.97%提高到46.76%,
提高了15,74倍。实验结果表明,D290树脂对莽草酸的吸附量大,脱附容易,可以应用于莽草酸的分离纯化。
关鍵词:离子交換树脂;白扦;莽草酸;分离;纯化
中国分类号:TQ026.5文献标识码:A文章编号:1000-6613(2009)01-0145-0
Separation and purifcation of shikimic acid from Picea meyeri with ion
exchange resin
YANG Lei, LU Yu, ZHANG Lin, ZU Yangang
(Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
Helongtiang, China
Abstract: The process parameters of the separation and purification of shikimic acid from Picea meyeri
with ion exchange resin were established. Eight different kinds of ion exchange resins named D261
D296, D301-R, D301-G, D290, 201*7(717), D201 and D280 were studied and the static capacity ol
adsorption and desorption with shikimic acidcwas investigated. The better resin was D290. The
optimum adsorption and desorption parameters of separation and purification shikimic acid with resin
D290 were as follows, adsorption: PH 6, 25C, flow rate 3 ml/min; desorption: eluent 2.5% NAOH
queous solution, flow rate 3 ml/min After treating with D290, the yield of shikimic acid was 92.53%
and the purity increased from 2.97% to 46.74%. 15.74 times as high as the crude extract. The results
showed that resin D290 had good ability of separation and purification of shikimic acid
Key words: ion exchange resin: Picea meyeri; shikimic acid; separation; purification
莽草酸为一种白色结晶性粉末,易溶于水,难こ,并且.30kg八角茴香只能加工出1kg莽草酸,
溶于氯仿、苯和石油醚;具有抗炎、镇痛作用,通因此寻找新的莽草酸资源变得极为重要。国内学者
过影响花生四烯酸代谢,可抑制血小板聚集,抑制检测到近20种针叶植物中含有莽草酸?,白扦(Pic
动、静脉血栓及脑血栓形成。自禽流感疫情受到世 meyeri Rehd. Et Wils)是中困特有的裸子植物?,常
界的普遍关注后,治疗其特效药物磷酸司米韦
( Oseltamivir Phosphate)日益受到重视,而合成这收稿日期:200-06-18:修改稿日期:208-07-16
基金项日:国家科技支撑计划项?(2006BAD18B04)、国家林业局重
种药物的重要中间体就是莽草酸2?。传统的制各工点科学技术研究项目(2053东北林业大学青午科研基金项目
艺都是从八角茴香中提取分离莽草酸的?,但由于八(070?1)资功。
角苗香在全球仅少数地区オ有分布,在我国只有4第一作者简介?杨(1964-),男,副教援,士要从事天然产物的
提取分离和应用的研究工作。联系人:祖元刚,教授,博士生导师
个省份生长,通常只在3~5月收获剧,资源极度缺
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