多叶越南槐中苦参碱与氧化苦参碱的分离鉴定及含量测定研究.pdf

广画ほ学2014年8月第36卷第8期
多叶越南槐中苦参碱与氧化苦参碱的分离鉴定及含量测定研究
陆国寿叶勇2卢文杰3黄周锌曾繁强
(1广西中医药研究院,南宁市530022,E-mail:luguoshou@foxmail.com;2广西医科大学,南宁市530021
3广西中药质量标准研究重点实验室,南宁市530022)
摘要】目的对多叶越南槐中苦参碱与氧化苦参碱进行分离及含量测定。方法采用溶剂提取、萃取、硅
胶色谱柱分离纯化,利用色谱技术进行化学结构鉴定;高效液相色谱(HPIC)法色谱条件为: Kromasil1005NH2
(250mmx4.6mm,5wm)色谱柱;流动相为乙腈一磷酸二氢钠(0.05mol/L)-磷酸水溶液(3.0%)-乙醇
(80:5:9.5:5.5);流遠为1.0m/min;检测波长为210nm;柱温为30℃。结果从多叶越南槐中分离得到两
生物碱类化合物,经鎣定为苦参碱与氧化苦参碱;苦参碱在0.0164~0.1476g范国与其峰面积呈良好的线性
关系,氧化苦参碱在0.2872~2.58488范国与其峰面积呈良好的线性关系。苦参碱和氧化苦参碱的加样回
收率分别为100.2%(RSD=0.76%)99.8%(RSD=1.19%)。结论苦参碱与氧化苦参碱均为首次从多叶
越南槐中分离得到;所建立的HPLC简便、准确、重现性好,结果准确可靠,可用于多叶越南槐中苦参碱与氧化
苦参碱的含量测定
关键词)】多叶越南槐;苦参碱;氟化苦参碱;高效液相色谱;含量测定
中图分类号】R284.1【文献标识码】A【文章编号】0253-4304(2014)08-1109-04
DOL:10.11675/j.1isn.0253-4304.2014.08.28
Isolation, Identification and Quantitative Analysis of Matrine and
Oxymatrine in Sophora tonkinensis Gagnep. var. polyphylla
LU Guo-shou", YE Yong, LU Wen-jie:, HUANG Zhou-feng, ZENG Fan-qiang?
(1 Guangxi Institute of onl Medic and Phamaceutical Science, Nanning 530022, China; 2 Guangxi Medical University
Nanning 530021, China; 3 Guan xi Ke Lborao of Td Chinese Medcine uli Standards, Nanming 530022, China
( Abstract] Objective To study the isolation and assaying of matrine and oxymatrine in Sophora tonkinensis
Gagnep. r Polyh la. Methods The chemical constituents were extracted by solvent and then isolated by silica
gelchromatographycolumn. The chromatographic technique was used to identify its chemical structures. The chromatographic
conditions of assaying by high performance liquid chromatography(HPLC) as follows: Kromasil 100-5 NH, column
(250 mm 4.6 mm, 5 wm with aceoniile-mome li m posphate(. 5 mo/)-phosphoric acid
solution(3.0%)-ethylalcohol (80: 5: 9.5: 5.5 as mobile phase, the flow rate was 1.0 ml/min, the UV detection
wave length was 210 nm, and the column temperature was 30C. Results The two alkaloids compounds attained from
the separation of Sophora tonkinensis Gage. war. Pohl were identified as main and matrine. The matrine
formed linear relation with its peak area when it was in the range of 0. 0164-0. 1476 wg. The oxymatrine formed
linear relation with its peak area when it was in the range of 0.2872-2. 5848 wg. The average recovery rates of
matrine and oxymatrine were 100. 2%(RSD=0. 76%)and 99.8%(RSD=1. 19%), respectively. Conclusion
Matrine and oxymatrine are attained from the separation of Sophora tonkinensis Gagnep. war. Polyphylla for the first
time. The established method( HPLC in this study is simple, reliable, reproducible and accurate. It could be applied
to the assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep. war. polyphylla
Key words Sophora tonkinensis Gagne. war. Polyphylla; Matrine; Oxymatrine; High performance liquid
chromatography; Assaying
▲基金项目:广西自然科学基金资助项目(2010 GXNSFA013135);西科学研究与技术开发计划项目(11100903-4
作者简介:陆国寿(1980~),男,本科,助理研究员,研究方向:中药化学
通讯作者:叶勇(1979~),男,博士,讲师,研究方向:民族药物的研究开发,E-mail:yong-ye?163.com。