NaCl盐析法从猪血粉水解液中提取L-亮氨酸.pdf

化工进展
2009年第28卷第3期
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
481
centere
研究开发
NaCI盐析法从猪血粉水解液中提取L亮氨酸
翁连进2,韩媛煖',郭旭
华侨大学化工学院,福建厦门361021:2泉州市泉港南元化工有限公司,福建泉州362811)
摘要:以NaCI为盐析剂从猪血粉水解液中盐祈沉淀レ亮氨酸,确定了盐析沉淀所需的时间,考察了温度、pH
值、NaCI加入量等因素对L-亮氨酸盐析沉淀率的影响。结果表明:盐析剂NaCl在原料液全溶解后搅拌半小时盐
析沉淀率基本上达到最大值;低温对于盐析沉淀是有利的,工业生产中可在室温下或低温下进行;在溶液的pH
值约为2时,NaI存在下L亮氨酸以二亮氩酸盐酸盐形式析出,沉淀率达到最大;溶液中氟化钠浓度越高盐析
沉淀率越高,工业生产上可按0.35g/mL的比例加入NaCl.室温下控制猪血粉水解液的pH值为2,按0.35g/mL
原料液的比例加入NaCI盐析出二亮氨酸盐酸盐,经精制得L亮复酸产品,产品符合《中华人民共和国药典》(2000
年版二部)质量标准,L亮氨酸的平均提取率为82.36%
关键词:氯化钠;盐析;L亮氨酸;提取
中图分类号:TQ425.9文献标识码:A文章编号:1000-6613(2009)03-0481-04
Extraction of L-leucine from hydrolysate of pig blood powder by Nacl
salting-out method
WENG Lianjin", HAN Yuanyuan, GUO Xu
( College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian, China; Quangang Nanyuan
Chemical Co Ltd, Quanzhou 362811, Fuiian, China)
Abstract: L-leucine can be deposited from hydrolysate of pig blood powder when sodium chloride
(Nac)is used as salting-out agent. In the deposition process, the time of salting-out deposition was
determined, and influence of the main factors, such as temperature, PH value, and Nacl quantity on
deposition efficiency was investigated. The results show that salting-out Nacl in the feed liquid. A low
temperature was good for salting-out deposition, so industrial process can carried out at room
temperature or low temperature. L-leucine is deposited as dileucine salt and the deposition efficiency
reached a maximum when PH value of the solution is about 2 and Nacl was present. The greater the
Nacl concentration in the solution, the higher the deposition efficiency. Addition of Nacl was 0.35 /ml
in the industrial extraction process
Key words: sodium chloride; salting-out; L-leucine; extraction
L-亮氨酸是人与动物的八大必需氨基酸之一,在甲苯-4-磺酸能与-亮氨酸形成微溶性沉淀的特点
医药、食品领域有广泛的应用叫。L-亮氨酸大量存在将L亮氨酸从蛋白水解液中分离出来。邻二甲苯-4
于如人发、猪毛、猪血粉、棉籽柏等废帘蛋白质中。
这些废弃蚩白质在我国每年的产量巨大且价值低收稿日期:2008-09-06:修改稿日期:2008-11-05
廉,从中提取亮氨酸仍具有艮好的经济效益。
基金项目:福建省科技计划重点项目(2007H0025)及泉州市科技计
划重大项目子项目(2005ZD45)资助
T业上从蛋白质水解液中提取亮氨酸目前第一作者简介。连进(9-)男,博士,教授。电活055-
主要是邻二甲苯-4磺酸沉淀法24,它是利用邻
22693828:E-mailljweng6907@163.coma