高效液相色谱-串联质谱法同时测定婴幼儿配方食品中香兰素、甲基香兰素和乙基香兰素.pdf

第5卷第11期
食品安全质量检测学报
Vol 5 No. 11
2014年11月
Journal of Food Salcly and Quality
2014
高效液相色谱-串联质谱法同时测定婴幼儿配方
食品中香兰素、甲基香兰素和乙基香兰素
肖锋,张殺,陈沛金,侯乐锡,易冰清,岳振峰
(深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室,深圳518000
摘要:目的建立了高效液相色谱-串联质谱法同时测定婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的
方法。方法样品经Iris溶液和乙腈振荡提取,离心后提取液用正己烷脱脂过滤后上机測定,外标法定量。
结果3种化合物在5.0~200gL范围内线性良好,在液态奶中测定低限均为25gkg,在奶粉中测定低限均
为50gkg,方法加标收率在89.5%6~115%之间,相对标准偏差(RSD小于15.6%结论该方法简便、快速,准
确,灵敏度及精密度高,适用于婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的测定。
关键词:婴幼儿配方食品;香兰素;甲基香兰素;乙基香兰素;高效液相色谱-串联质谱法
Simultaneous determination of vanillin, methyl vanillin and ethyl vanillin in
infant formulas by high performance liquid chromatography-tandem mass
spectrometry method
XIAO Feng, ZHANG Yi, CHEN Pei-jin, HOU Le-xi, YI Bing-qing, YUE Zhen-feng
(henzhen Key laboratoy of Detection techmology Rc 1 on Tood Safety, Pood inspection and arantine Center, henzhen
Entry-exit Inspection and Quarantine Bureau, Shenzhen 518000, China)
ABSTRACT Objective A method is presented for the quantitative determination and con firmation of vanil
lin, methyl vanillin and ethyl vanillin in infant formulas by high performance liquid chromatography-tandem
mass spcctromctry(hplc-ms/MS). Methods Samples were extracted by Tris solution and acetonitrile. The
supernatant was centrifuged and delated by n-hexane before the 0.22 wm filter apparatus was used, Tinally de
tected by TPT C/MS/MS and quantitated by external standard method. Results good linearity of three
compounds was obtaincd in the range of 5.0-200 ul. Ihe limits of detection of three compounds werc 25
uy/kg in liquid milk and 50 ug/kg in milk powder. Mean recoveries of three compounds ranged from 89.5% to
115%. The relative standard deviation was less than 15.6. Conclusion The method was proved to be a sim-
ple, rapid, accurate and sensitive method and was successfully applicd to detect vanillin, methyl vanillin and
ethyl vanillin in inlant Iormula
KEY WORDS: in lant formula vanillin; methyl vanillin; ethyl vanillin; high performance liquid chromato-
eraphy-tandem mass spectrometry
基金项目:国家科技支撑计划项目(2012BAKO8B01)深圳市基础研究项目(JCY20111097、JCYJ201206187214417
Fund: Supported by the National Key Techology R&D rogram(2012B/K08B01 )and Shenzhen Basic Rcscarch Plan Projcct(JC Y201110097
JCYJ2012061872144497
通讯作者:肖锋,工程师,主要研究方向为食品安全检测。T-mai:xiaofeng2003(163.com
Corresponding author: XIA Fcng, Enginccr, Food Inspcction Ccnter of Ciq-shcnzhcn, Shenzhen Entry-exit Inspection and Quarantino
Burcau,NO.1011,FugiangRoad,FutianDistrict,Shcnzhen518045,China.E-mail:mxiaofcng2003(0163.com