奶粉中香兰素、甲基香兰素和乙基香兰素的测定.pdf

食品研究与开发
2015年7月
检测分析
Food Research And Development
第36卷第13期
DOI:10.3969/.issn.1005-6521.2015.13.022
奶粉中香兰素、甲基香兰素和
乙基香兰素的测定
籍芳,孙玉慧,李天宝,王春利,刘炜,李文斌
(1.江门出入境检验检疫局,广东江门5290002.北京迪马科技有限公司,北京100000
摘要:建立了同时测定奶粉香兰素、甲基香兰素和乙基香兰素的超高效液相色谱法。样品用水溶解后使用 Proelut
PXA固相萃取柱进行样品前处理净化,C1s柱分离,超高效液相色谱仪进行色谱分析。结果表明,香兰素、甲基香兰素
和乙基香兰素在0.5mg/L~20mgL范固内线性关系良好;方法的检出限(SN=3)分別为0.20、0.25、0.25mgL;添加水
平在25mg1.~-10.0mgy.时加标回收率为80.3%~102.7%,相对标准偏差为1.85%~4.119%(n=6)。该方法结果准确可
靠,可实现奶粉中香兰素、甲基香兰素和乙基香兰素的快速同步分析
关键词:固相萃取;超高效液相色谱;妈粉;香兰素;甲基香兰素;乙基香兰素
Determination of Vanillin, Methyl Vanillin and Ethyl Vanillin in Milk Powder
JT Fang, SUN Yu-hui , TI Tian-bao, WANG Chun-li LIU Wei,I I Wen-bin
(1. Jiangmen Entry-exit Inspection and Quarantine Bureau, Jiangmen 529000, Guangdong, China
2. Dikma Technologies Incorporation, Beijing 100000, China
Abstract: An ultra performance liquid chromatography (UPLC)method was developed for the simultaneous
determination of vanillin, methyl vanillin and ethyl vanillin in milk powder Samples were dissolved with water
purified with solid phase extraction column, separated with Cis column, and determined with ultra performance
liquid chromatography. The results showed that there were good linear relationships between peak areas and
concentrations of vanillin, methyl vanillin and ethyl vanillin in the range of O5 m/1-20 mg/, with limits of
detection of 0.20 mg/L, 0.25 mg/L and 0.25 mg/L, respectively. The recoveries at spiked levels of 2.5 mg/L
10.0 m/L were in the range of 80.3%-102.7, with the relative standard deviations (RSDS, n=6)of 1.85
4.11 c. The method was sensitive and reliable. and could achieve the fast simultaneous analvsis of vanillin
methyl vanillin and ethyl vanillin in milk powder
Key words: solid phase extraction; Ultra performance liquid chromatography; milk powder; vanillin; methyl
vanillin; ethyl vanillin
香兰素、甲基香兰素和乙基香兰素均是重要的食併十、面包和炒货等食品用量居多,另外还可做饲料
用香料,用途十分广泛,通常在食品、口化、烟草工业中的添加剂、电镀行业的増亮剂、制药行业的中间体。研
作为香原料、矫味剂或定香剂,其中饮料、糖果、糕点、究表明,大剂量食用可导致头痛、恶心、呕吐、呼吸困
难,甚至损伤肝、肾。根据CB2760-2011《食品安全国
基金项目:)东省科技计划项目(2011BO10400019);国家质检总局
家标准食品添加剂使用标准》要求,凡使用范围涵盖0
科技计划项目(20141K169)
作者简介:籍芳(1979-),女(汉)、工程师,硕1研究生,主要研究方至6个月婴幼儿配方食品,不得添加任何食用香料。
向:进出?商品檢验。
日前,文献报道测定香兰素的方法有紫外分光光
通信作者:李天宝(1981ー),男(汉),高级工程师,倾上研究生
度法、高效液相色谱法、电化学法門、荧光分光光
要研究方向:进出?商品檢验。
度法气相色谱-质谱联用法但鲜见固相萃取结