泽兰与小茴香提取物抑制大鼠前列腺增生的实验研究.pdf

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医学研究生学报2014年12月第27卷第12期 J Med Postgra,Vol.27,No.12, December,2014
论
著
(基融研究
泽兰与小茴香提取物抑制大鼠前列腺增生的实验研究
唐维,代光成,薛波新,单玉喜,张文芳
[摘要]目的良性前列腺增生症( benign prostatic hyperplasia,BPH)的新药研发是近年来的研究热点。文中研究泽兰
与小茴香提取物(中药复方)对大鼠前列腺增生模型的影响及探讨其作用机制。方法48只雄性大鼠按随机列表法分为6
组:正常组,模型组,度他雄胺组,中药复方组高、中、低剂量组(312、234、156mg/kg),每组8只,正常组乙醚麻醉后无菌游离双
侧睾丸及附睾,但不切除睾丸。其余组麻醉后切开阴囊皮肤,切除双侧睾丸及附睾,开始造模,皮下注射丙酸峯酮0.5mg/0.02
mL,1次/d,连续14d。中药复方剂量组按上述剂量灌胃给药,正常组及模型组灌胃给予等体积蒸馏水,1次/d,连续45d。观
察每组给药45d后大鼠前列腺混重、前列腺指数、前列腺组织形态学变化及性激素水平。结果中药复方高、中、低剂量组
前列腺湿重[(1.766t0.245)、(1.8120.28)和(1.872t0.328)gl和前列腺指数「(0.441±0.069)%、(0.4650.078)%和
(0.481±0.085)%]较模型组[(2.284±0.241)、(0.586±0.100)%]降低(P<0.01);中药复方高、中、低剂量组血清睾酮含
量[(0.18±0.01)、(0.20±0.01)、(0.23±0.02)ng/m]较模型组[(0.24±0.03)ng/m]明显降低,双氢睾含量[(5.46±
10.92)、(6.21±9.12)、(67.39±8.88)nmoM/I]较模型组[(80.48±11.55)nmol/L!明鼠降低,睾酮/雌二醇偵(137.25±
19.64、152.34±17.47、16667±15.42)较模型组(175.24±27.32)明显降低,差异均有统计学意义(P<0.05)。中药复方大剂
量组与度他雄胺组大鼠前列腺腺体少量增生,腺上皮乳头减少或消失,腺体上皮细胞呈低立方或扁平状,大小较为一致,排列
整齐,接近正常腺体。结论泽兰与小茴香提取物能显著抑制大鼠前列腺增生,其作用机制在一定程度上与降低大鼠血清
睾酮、双氢睾酮含量及睾酮/雌二醇值有关。
[关键词]良性前列腺增生;泽兰;小苗香;性激素
[中图分类号]R69
文献标志码
[文章编号]1008-8199(2014)12-126603
The inhibitory effect of eupatorium japonicum thunb and foeniculum vulgare extract on prostatic hyperpla
sia in rats
TANG Wei, DA Guang-cheng, XUE Bo-xin, SHAN Yu-xi ZHANG Wen-fang
1. Department of Urinary Surgery, the Second Afiliated Hosital of Soochow University, Soochou 215004 iangsu
China 2. Soochou Nanocrystal Pharma Co, LD, Soochow 215123, Jiangsu, China
I Abstract Objective Benign prostatic hyperplasia(BPH)is one of common diseases in aged males, and searching for new
therapeutic drugs to BPH has been a research hotspot in recent years. This article was to study the inhibitory effect of eupatorium ja
panicum thunb and foeniculum vulgare extract(EFE)on benign prostatic hyperplasia in rats and its possible mechanism. Methods
48 male rats were randomly divided in 6 groups: normal control grou without any rea ment, model group of BPH treated with subcu
taneous injection of testosterone propionate, positive control group of BPH treated with dutasteride, high, middle and low dosage groups
according to different EFE dosage(156 m/kg, 234 mg/kg and 312 m/kg). 45 days after the treatment, the rats were sacrificed for
measurement of the prostate glandular wet weight, the index of prostate gland (PI), the morphological changes of prostate gland by
light microscopy and the content of sex hormone. Results The prostate wet weight and PI decreased after EFE treatment for 45 days
compared with the BPH model group(P<0.1 The hyperplastic glandular epithelium papilla waned and even disappeared in three
EFE groups under the light microscope, and the epithelial cells became cubical or flat. High dosage EFE group(312 m/kg)has simi
lar efficacy to dutasteride group. EFE significantly reduced eum testosterone content, dihydrotestosterone content and T/E2 ratio(P
0.05
can s
基金项目:江苏省科技支撑计划-社会发展项目(BE011671
hyperplasia in rats, and its mechanism is related to
作者单位:215004苏州,苏州大学附属第二医院泌尿外科
the contents of serum testosterone and dihydrotestosterone as well as
唐维(医学硕士研究生)、代光成、薛波新、单
T/E2 ratio
玉喜1;215123苏州,苏州纳晶医药技术有限公司
I Key words Benign prostatic
Eupatorium
(张文芳
Japonic umn
通讯作者:薛波新,E-mail:xbxcp@hotmail.com
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