水酶法提取葡萄籽油和蛋白质的研究.pdf

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CHINA OILS AND FATS
2017Vol.42No.9
油脂加エ
水酶法提取葡萄籽油和蛋白质的研究
李丹丹,杨宏志,梁英,朱磊
(黑龙江八ー农垦大学食品学院,黑龙江大庆163319)
摘要∷为充分利用葡萄酿酒后的副产物,对水酶法提取葡萄将中的油脂和蛋白质的工艺进行优化,
并研究不同干燥方法和提取方法对葡萄籽油提取量和理化性质的影响。结果表明∷最忧提取条件
为纤维素酶、果胶酶、木聚糖酶、胰蛋白酶质量比2121、pH6.5、料液比19、酶解时间9h,在此条
件下,葡萄籽油提取量为99.47mg/g,蛋白质提取量为74.86mg/g,蛋白质提取率为57.58%:不同
干燥方法对水酶法提取葡萄籽油的提取量和理化性质影响显著,从油的提取量、品质和成本考虑,
在生产中应优先选择风干的千燥方式与传统方法相比,水酶法提取的葡萄籽油品质更好,并可以
直接得到副产物蛋白质,但是提取效率不够理想,有待进一步研究。
关键词:水酶法:葡萄籽油;蛋白质∵提取量理化性质
中图分类号:TS224.4:TS229文献标识码:A
文章编号:1003-7969(2017)09-0028-06
Aqueous enzymatic extraction process of oil and protein from grape seeds
LI Dandan, YANG Hongzhi, LIANG Ying, ZHU Lei
College of Food Science and Technology, Heilongjiang Bayi Agricultural University,
Daqing 163319, Heilongjiang, China
Abstract: In order to make full use of byproducts of grapes after brewing, the extraction processes of oil
and protein from grape seeds by aqueous enzymatic method were optimized, and the effects of different dr-
ng and extraction ways on the extraction amount and physicochemical properties of grape seed oil were
investigated. The results showed that the optimal extraction conditions were obtained as follows. mass ratio
of cellulose, pectinase, xylanase and trypsin 2 1 2 PH 6.5, ratio of material to liquid I and enzy
molysis time 9 h. Under these conditions, the extraction amount of grape seed oil, extraction amount and
extraction rate of protein were 99.47, 74.86 mg/g and 57.58%, respectively. Different drying ways had
obvious influences on the extraction amount and physicochemical properties of grape seed oil. Considera
tion from extraction amount of oil, quality and cost, air drying should be preferred in production. Com
pared with traditional methods, the quality of grape seed oil extracted by aqueous enzymatic method was
better, and the protein byproducts could be obtained directly. However, the extraction efficiency was not
ideal enough, which need further studies
Key words aqueous enzymatic method, grape seed oil, protein, extraction amount, physicochemical property
葡萄籽中含有大量的脂类、蛋白质和多酚类物质"。葡萄籽油占葡萄籽质量的14%~17%,含有
大量以亚油酸为主的不饱和脂肪酸、植物甾醇和白
收稿日期2017-03-14'修回日期:2017-05-23
藜芦醇等生物活性成分,还含有多种维生素和矿物
基金项目:国家自然科学基金青年科学基金项目(3101828);:质2。研究发现,葡萄籽油可以清除沉积的血清
大庆市指导性科技计划项目(xd-2016-108);黑龙江八ー农胆固醇,促进前列腺素合成阳,还有营养脑细
垦大学引进人オ科研启动计划资助项目(XYB2013-16)
胞、调节神经功能等作用。葡萄籽油主要应用于
作者简介:李丹丹(1992),女,硕士研究生,研究方向为天然食品营养、医疗保健以及日用化汝品等领域。葡萄
产物的活性研究(E-mail)dandan.349382650@hotmail.com。
籽制油后的饼粕中含有13%~16%的蛋白质,富
通信作者:梁英,教授,博士(Fmui) liangying-64163.含18种氨基酸,包括人体必需的8种氨基酸,是
com;朱磊,讲师,博士(Emai)zhuli610@163.com
一种优质蛋白质资源,因此可利用葡萄将蛋白生产
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