重组木聚糖酶生产低聚木糖的实验研究.pdf

第31卷第2期
林
化学与工业
Vo⊥31No
2011年4月
Chan istry and Industry of Forest Products
Apr 2011
重组木聚糖酶生产低聚木糖的实验研究
欧阳嘉,刘明,李鑫,陈牧,连之娜
(南京林业大学化学工程学院,江苏南京210037
摘要:研究了重组木聚糖酶C0602生产低聚木糖的制备工艺,考察了不同类型底物、底物浓度
和酶用量对酶解效率和低聚木糖产卒的影响。实验结果表明,低聚木糖生产原料以抽提木聚糖为
宜。桦木木聚糖质量浓度30g重组木聚糖酶用量20U/g条件下水解4h酶解率可达
OUYANG ia
5605%,低聚木糖产率(C2C6)为2976%,产品平均聚合度为2767。245g/L碱抽提玉米芯木
聚糖在酶用量80Ug条件下水解48h酶解率6833%,低聚木糖产率为14-88%。产品不含木糖,平均聚合度为345
利用重组木聚糖酶生戸产低聚木糖可以大幅度降低产品中的木糖含量,显著提高低聚木糖产品质量
关键词:木聚糖酶;低聚木糖:酶水解;聚合度
中图分类号:TQ351
文献标识码:A
文章编号:0253-24172011)02-0037-06
Experim en l Iinvestigation of Xy loo ligosaccharides Production b
Recam binant Xy lanase
OUYANG Jia「 TU M ing「. IX in CHEN Mu LTAN Zhina
(The Collge ofchem ical Engnccring N an jng F ores ry U n icrsity N anjing 210037, China
Abstract y lool gosaccharies(O)putin us ing the rec bian xy lan ase C0602 w as stud ed E fects of d iferent sub
st ate st e onct on an en ae n the enzym atic yd ys is and xy ol gosa harie y ie were nvest ga tel
The resu s how that the ext acted xy lan was he su itab E substrate o r xy bo ligosacchar iles production W hen 30 g/L b Iich
w od xylan was treated w ith he dosage of 20 U /g xy lease forhydmo lysis 4 h the hydro bs i yield was 5005% and he xy loligo
saccharine yield (c2-c6)was 29. 76. The average degre of po m erization(Dp ) was 2 76 W hen 24 5 g/L xylan
ted fron comcob w as hydro zed by 80 /g xy anase for 48 h the hym ys i yic rcached 6 33%and he xy o igosacchar ile
yicld w a 14 88 orson nly the pructdid not co ta in xy bse and its DP was 3 45 The results show e that xy lool go
saccharine prluctin using he reom binant xy lanas could greatly decrease the content of xy bse and significantly in prove the
qua lity of xy bo ligosaccharides
K ey words xy lanas xy loo gosaccharilg zym atr hydro lysis degree of polm er ization
低聚木糖,亦称木寡糖,是由28个木糖以14糖苷键连接而成的低度聚合糖的总称,共中以
木二糖和木三糖为主。近年米由于低聚木糖在人或动物体内找不到札相应的代谢晦系,尤法作为能量
体,但却可以发挥以增体内双歧杆菌为主的众多保健功能而受到人们的广泛认可2?。低漿木糖的
牛产?前主要以嗨法水解木聚糖为主。天然产木聚糖海一般均包括内切木聚糖嗨和利外切木糖嗨
两种组分。前者从主链内部作川于木糖苷链,可随机将木聚糖主链降解成短链的低聚木糖,而后者作用
」氖链低聚木糖,从非还原性末端释放山出木糖。木聚料糖跡中木糖台海的存是低聚木糖产品木糖含星
较高,产品纯度和得率较差的主要原因。利用无木糖苷酶酶活的木聚糖酶生产低聚木糖表现出良
好的工业化前景,但研究报逆很少。本实验在前期工作中成功构建了不含水糖忏活的木聚糖
收稿日期:2010-09-25
基金项目:家自然科学基金资助项日(31070513):家重点基础研究发展词划(973)资助项日(2007CB707801);江苏省高校自然
科学斫究大项日(10KIA22019)
作者简介:欧附嘉(1972-),女,湖南湘潭人,副教授,博上,上生导师,主契从事牛物质能源开发利川和晦工程研究工作
E-mail hgouyfa nfu eu. crh
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