凝胶渗透色谱-高效液相色谱法测定蔬菜中呋喃丹的含量.pdf

第7卷第5期
食品安全质量检测学报
Val. 7 No 5
2016年5月
Joumal of Food Safety and Quality
May,2016
凝胶渗透色谱-高效液相色谱法沏定蔬萊中
呋喃丹的含量
冯晓青,汪怡,王芹,王露,宋鑫,杭学字
淮安市疾病预防控制中心,淮安223001
摘要:目的建立凝胶滲透色谱-高效液相色谱法检測蔬菜中的呋喃丹农药残囧量。方法样品经乙腈提取
和环己烷-乙酸乙酯溶解后,经凝胶渗透色谱净化和反相高效液相C1g色谱柱分离后,采用荧光检测器进行测定,
荧光检测激发波长为285nm,发射波长为320mm,采用高效液相色谱法以外标法定量。结果呋喃丹在
0.2~5.0 ng/ML浓度范围内呈良好线性关系,相关系数r大于0.99,检测限为0.01mg/kg。对空白蔬菜样品进
行呹喃丹3个浓度水平0.5、10和2.0mgkg的加标,回收率结果为78.3%~97.6%,相对标准偏差为
3.26%~1169%。结论本研究所建立的方法简单、灵敏度高,可适用于藐菜中的呋喃丹农药残留的分析检测。
关键词:高效液相色谱;呋喃丹;凝胶渗透色谱;蔬菜
Determination of carbofuran in vegetables by gel permeation
chromatography-highperformance liquid chromatography
FENG Xiao-qing, WANG Yi, WANG Qin, WANG Lu, SONG Xin, HANG Xue-yu
Control and Prevention Huai'an 223001 Chi
ABSTRACT: Objective To establish a method for determination of carbofuraninvegetablesby high performance
liquid chromato raphy( HPC with purification ofel permeation chromatography(G]C). Methods Samples wr
traced withaceonitile and dissolved with ethyl acetate-cyclohexanemixture, and then separated by reversed-phase
C column followed with GPC purification. The fluorescence excitation and emission wavelengths were 285 nm and
320 nm. Identification of carbofuran was performed by HPLC with an external standard method. Results
Carbofuranhad a good linear relationship in the range of 0.2-5.0 ng/ml with the correlation coefficient greater than
0.999. The limit of detection(LO D) was 0 Img/kg. The average recoveries at 3 spiked levels of 0.5, 1.0 and 2.0
mg/kg were ranged from 78.3% to 97.6%, and the relative standard deviations(RSD)were in the range of
3. 26%-11.69%. Conclusion The established method is simple and sensitive, which issui able forthe determination
of carbofuran in vegetables
KEY WORDS: high performance liquid chromatography carbofuran; elpermeation chromatography; vegetables
易急性中毒,在蔬菜生产过程被禁用。由于人为和其他
引言
原因,呋喃丹误用现象时有发生,国家食品安全风险监测
呋喃丹是一种内吸性杀虫剂,因其残留毒性高、口服计划将其作为硫菜和水果中氨基甲酸酯类其中一项监测指
通讯作者:杭学宇,副主任技帅,主要研究方向为理化检验。E-mail:664686545@qcom
Corresponding author: HANG Xue-yu, Associate Chief Technician, Huai an Center for Disease Control and Prevention, N. 118 Hu Road
Huai'an 223001, China. E-mail: 664686545@qq con
万方数据