肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC检测方法.pdf

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品字
2014,Wl.35,NO.16101
肉鸭表皮组织中脱氢枞酸残留的SPE-HPLC
检测方法
张苏珍'2,耿志明*,王道营2,诸永志,刘芳,张牧焓2,卞欢,徐为民2
(1.南京农业大学肉品加工与质量控制教育部重点实验室,江苏南京210095;
2.江苏省农业科学院农产品加工研究所,江苏南京210014)
摘要:建立经松香脱毛处理的肉鸭表皮组织中残留脱氢枞酸的固相萃取高效液相色谱检测方法。肉鸭表皮组织
中的脱氢枞酸用乙腈提取,经C:固相萃取小柱净化,以0.03mo阢L磷酸溶液-甲醇(13:87,W)为流动相,采用
反相C8(250mmX4.6mm,5um)色谱柱分离,以紫外检测器(检测波长212nm)进行检测。结果表明:该方法
检测脱氢枞酸线性范围为0.1~10mgL,检测限为0.10ug/g,定量限为033ug/g。在低中高3个添加量范围内回收率
为80.8%~91.8%。实际样品分析显示,肉鸭表皮中脱氢枞酸含量最高达10.06gg。所建立的分析方法简便快捷、
具有较好的灵敏度和可靠性,可用于肉鸭表皮组织中脱氢枞酸残留量的分析。
关键词:肉鸭;松香;脱氢枞酸;高效液相色谱法;固相萃取
Development of SPE-HPLC Method for Detemination of Dehy o enated Abietic Acid Residue in Duck Skin Tissue
ZHANG Su-zhen, GENG Zhi-ming", WANG Da in ZHU Yong-hi, LIU Fang, ZHANG M- an, BIAN Huan, XU wei-m
(1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University,
Nanjing 2 5 China 2. In of A l s P ig J Aca o A l S nes Naning 210014, China
Abstract: A high-performance liquid chromatographic(HP)method was established to determine dehydrogenated abietic
acid in duck skin. Dehydrogenated abietic acid in duck skin sample was extracted with acetonitrile, followed by purification
with a i solid-phase exraction(P a i e and de eed y Hplc-ulavilet detector(U). The chromatograp
conditions were as follows: column, reversed-phase (250 mm X 4.6 mm, 5 um); mobile phase, a mixture of0.003 mo/L
phosphoric acid-methanol(13: 87, flow rate, 1. 0 ml/min; and detection wavelength, 212 nm. Results indicated that
the linearity ranged from 0.1 to 10.0 m L, and the detection limit and the quan ification limit were 0.10 ug/g and 0.33 H
respectively. The recoveries of dehydrogenated abietic acid spiked at 0. 5-20 ug/g were in the range of 80.8%91.8%.The
method was successfully applied for the determination of dehydrogenated abietic acid in rosin-defeathered duck skin, and
the result indicated that content of dehydrogenated abietic acid in the duck sample could reach 10.06 ugg. The present
method proved to be of high maneuverability and excellent sensitivity and accuracy, and could be employed to analyze
dehydrogenated abietic acid residue in duck skin tissue
Key words: duck; rosin; dehydrogenated abietic acid; high-performance liquid chromatographic(HPLC); solid polymer
electrolyte(SPE)
中图分类号:TS201.5
文献标志码:A
文章编号:1002-6630(2014)16-0101-04
doi:10.7506pkx1002-6630-201416019
脱氢枞酸(亦称脱氢松香酸)是松香的主要成分之制作用。细胞毒理学研究表明脱氢枞酸对人体红细胞、
。松香中的枞酸和脱氢枞酸是人们熟知的肺和皮肤致多核白细胞有毒性作用。脱氢枞酸和枞酸一样,广泛
敏剂,可以导致职业哮喘和接触过敏,它们的氧化产物存在于纸浆、造纸厂废液"の以及药剂"]、化妆品"?和
被认为是重要的皮肤过敏源。枞酸和脱氢枞酸可以损伤工业品中叫,在食品包装材料中也存在有相当浓度的脱
水生生物的DNA活性,对水蚤的生长发育有一定的限氢枞酸和枞酸。脱氢枞酸和枞酸的分析方法主要包括
收稿日期:2013-08-28
基金项目:国家自然科学基金青年科学基金项目(31101312);江苏省农业科技自主创新资金项目(CX(13)3081
作者简介:张苏珍(1988-),女,硕土研究生,研究方向为食品科学。E-mail:xsdzsz@126.com
通信作者:耿志明(1965-),男,副研究员,硕士,研究方向为食品化学。E-mail:Amgen163.com
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