三氯化铝比色测定火棘总黄酮方法的系统考察.pdf

食品研究与开发
年3月
检测分析
Food Research And Development
第36卷第5期
89
DOI:10.3969/.issn.1005-6521.2015.05.023
三氯化铝比色测定火棘总黄酮方法的系统考察
酃又玉2,夏婷2,张育,游元,赵春芳,余龙江1
(1.华中科技大学生命科学与技术学院,湖北武汉430074:2.武汉轻工大学生物与制药工程学院,
湖北武汉4.30023)
摘要:探讨以芦丁作为对照品,建立三氯化铝(AICl3)比色法测定火棘总黄酮含量的最优条件。系统考察了对照
品、光照、反应时间及温度、显色剂AIC浓度及用量、体系pH、缓冲液用量等因素对总黄酮与AlCl显色反应的影
响。结果表明,AICI比色法测定火棘总贲嗣含量的最优条件为:1mI,适宜浓度的火梾提取液,依次加入2ml-pH54
的醋酸一醋酸納缓冲液,1mL质量浓度2%AlCl3液,70%乙醇定容至10mL并混匀,不避光条件下30℃反应12min,
285mm測定吸光度。该测定方法具有良好的重复性、稳定性、重现性,回收率
关键词:火棘;总黄酮;芦丁;MlCl2比色法
Systematic Investigation on Alcl; Colorimetric Determination of Total Flavonoids from
Pyracantha Fortuneana Fruit
YAN You-yu", XIA Ting, ZHANG Yu, YOU Yuan, ZHAO Chun-fang, YU Long-jiang
(1. School of Life Science &Technology, Huazhong University of Science &Technology, Wuhan 430074
Hubei, China; 2. School of Biological & Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan
430023, Hubei, China
Abstract Rutin was taken as reference substance and the optimal condition of aluminum chloride colorimetric
assay affecting the determination of total flavonoids from Pyracantha fortuneana extract was established. Several
parameters, such as the standard substance, light, reaction Lime and temperature, concentration and the
amounl of chromogenic agent, PH and buffer dosage thal aflecting the color reaction of tolal flavonoids were
investigated systematically. The results indicated the appropriate reaction conditions were as the following: 2 ml
2 % (m/m )Aluminum chloride was added Lo I ml pyracantha extract with specific concentration, after being
blended the reaction was carried out at 30 C for 12 min. then absorbance was measured at 285 nm. As a result
Statistical analysis showed the method had good repeatability, stability, reproducibility and high recovery rate
Key words: Pyracantha Fortuneana; total flavonoids; rutin; aluminum chloride colorimetric method
火棘( Pyracantha fortuneana)属于蔷薇科苹果亚科等已开发得相当成功,广泛应用于食品、药品及化妆
( Maloideae),是一种药食两用常绿野生灌木,在我国品叫等领域,而在中国大陆火棘整体开发力度不够,仅
共有7个种属,广泛分布于东南、西南和西北部,尤以以盆景,果酒四,果醋?以及果实粗提物低端应用及出
湖北神农架地区资源最丰富,年均产量在5万t以上叫。口,具有极大的开发潜力,市场前景相当广阔。植物总
火棘富含黄类等天然抗氧化成分,研究表明,黄酮黄的含量测定一般采用直接分光光度法、比色法、
类化合物具有增强记忆刚抗衷老?抗波劳?、消食健高效液相色谱(IPIC)法等H。直接分光光度法灵敏
脾四通便润肠、降血脂、抗菌、改善脂蛋白、保护心度低,比色法能提高测定灵敏度,足被《中国药典》所
肌及抗糖尿病等功效。目前,火棘资源在日本、韩国采用的最常见的总黄酮测定方法,其中又以NaNO
作者简介:鄅又卡(1975一),女(汉),讲师,博士,研究方向:大然AI(NO3)-NaOH比色法最常用,但NaNO2-AI(NO2)
药物。
NaOH比色法不能排除邻二酚羟基非黄酮类物质
冰通信作者
干扰P?,而火棘中已经报道的很多非黄酮成分如鞣