酶比色法测定禽蛋中的胆固醇含量.pdf

第8卷第8期
食品安全质量检測学报
Vol. No. 8
2017年8月
Journal of Food Safety and Quality
Aug.,2017
嗨比色法测定禽蛋中的胆固醇含量
马丽娜,葛庆联,陈大伟,陆俊贤2,刘菌菌2,蒲俊华2,
丁红梅·2,高玉时
(1.江苏省家禽科学研究所,扬州225125;2.农业部禽类产品质量安全风险评佔实验室,扬州225125
摘要:目的建立晦比色法快速測定禽蛋中胆固醇含量的分析方法。方法以嗨比色法与分光光度计联用.
在波长505nm处直接测定经皂化、提取、浓缩和酶工作液催化后的禽蛋样品中胆固醇含量。结果本试验考
察了酶比色反应的最优显色温度(37℃)和显色时间(15min),并在最优试验条件下测得线性范固为0~2.0
mg/ml,曲线回归方程Y=0.3207X+0.0891,相关系数R为0.9990,检出限为1.0mg/100g,回收率达到
90.43%~98.12%,相对标准偏差小于2.45%。结论本方法操作简单、具有很好的精密度与准确度,适合鸡蛋
甲鸟貨、鹅蛋、鸽蛋等全蛋样品或蛋黄样品中胆固醇含量的快速检测。
关键词:禽蛋;胆固醇;酶比色法;分光光度计
Determination of cholesterol in eggs by enzyme colorimetry
MA LI-NA=, GE Qing-lian=, CHEN Da-wei=, LU Jun-xian", LIU Yin-yin", PU Jun-hua
DING Hong-mei, GAO Yu-shil2"
of Quality and Safety Risk
Assessment for Pouty Products, Minist of agriculture, Yangzho 225125, China
ABSTRACT: Objective To establish a method for determination of cholesterol in eggs by enzyme colorimetry
Methods Determination of cholesterol in eggs by enzyme colorimetry coupled with spectrophotometer at 505 nm
aller samples wcrc prcparcd by saponification, cxtraction, conccntration and cnzymc calalyzed rcaction. Results
The optimal coloration Lcmpcraturc (37 C)and coloration timc(15 min )had bccn invcstigatcd. The mcthod had a
linear range of calibration curve of 0-2.0 mg/ml for determination of cholesterol in eggs, and the linear correlation
coefficients were more than 0.999. The detection limit were 1.0 mg/100 g and the relative standard deviations(RSD
were not more than 2. 45%. The recoveries for the spiked samples ranged from 90.43% to 98.12%. Conclusion This
method is simple and accurate, which is suitable for the determination of cholesterol in egg, duck egg, goose eg
pigeon cgg and other wholc cgg samples or cgg yolk samples
KEY WORDS: cggs; cholesterol; cnzymc colorimetry; spcctrophotomclcr
基金项目:2017国家农产品质量安全风险评估重大专项-畜禽产品质量安全风险评估( GJIP2017007)、江苏省农业三新に程项目
Fund: Supported by Major Projects o! 2017 National Agricultural Product Qualily and Salcty Risk Assessmenl-animal Products Quality and
Salely Risk Assessment(G. FP2017007)and Agricultural Sanxin Projeet of Jiangsu Province(XGC2016292
通讯作者:高玉时、博士,研究员,主要研究方向为遗传育种与食品安全。E-mail:gaoyi00asina.com
*Corresponding author: (A Yu-shi, h 1, Professot, Jian su Institute of Poultry Sciences, No58, Cangjie Road, Hanjiang District Yanghou
25125,China.E-mail:gays100(@sina.com
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