姬松茸菌株J1与J77镉富集差异及生理响应机制.pdf

2017.36(5):863-868
农业环境科学学报
2017年5月
Journal of Agro-environment Science
刘朋虎,李波,江枝和,等.松茸菌株」与J镉高集差异及生理响应机制农业环境科学学报,2017.36(5):863-868
LIU Peng-hu, LI Bo, JIANG Zhi-he, et al. omparison and phsiol cal m anss of e mium(Cd a umulation y strain J and mutant Ja of Agricus
brasiliensis . J Joumal ofagro-hnhironmenr Science, 2017, 36(5): 863-868
姫松茸菌株J与Jm镉富集差异及生理响应机制
刘朋虎,李波2,江枝和,王义祥,翁伯琦
福建农林大学国家菌草工程技术研究中心,福州3.50002:2.福建农林大学资源与坏境学院,福州350002;3.福建省农业科学
院上壤肥料研究所,福州350013;4.福建省衣业科学院农业生态研究所,福州350013)
摘要:以烛松茸菌株J及其诱变菌株Jn为材料,研究不同浓度镉对」、J菌丝生长与产量、镉富集量及相关氨基酸含量等的影
响,分析J、Jn对镉吸收与耐受性差异及生理响应机制。结果表明:随着外源添加镉浓度的增加,JJn菌丝生长速度、产量均先增加
后降低,低浓度镉能促进姬松茸生长、提高产量;相同浓度外源镉胁迫下,Jm菌丝及子实体中镉含量均低」J1,且差异显著(P
0.01),Jn为低富集的菌株:菌丝中镉含量与子实体中镉含量显著正相关(R0.97):J1、Jn子实体中半胱氮酸含量均先降低后増
加,低浓度镉胁迫下J半胱酸含量较高,高浓度镉胁迫下Jn中半胱酸含量较高,半胱氨酸可能与松茸镉富集密切相关
关键词:姬松茸;镉;富集;重金属
中图分类号:X171.5文献标志码:A文章编号:1672-2043(2017)05-(0863-06doi:l0.1654/acs,2017-0073
Comparison and physiological mechanisms of cadmium(Cd )accumulation by strain J and mutant Jn of Agar
icus brasiliensis
LIU Peng-hu. LI Bo, JIANG Zhi-he, WANG Yi-xiang, WENG Bo-qi
(I. National Engineering Researh enter of JUN O Technology, Fujian re and Forest Uniersity, Fuzhou 350002 China; 2. ol
lege of Resources and Environment, Fujian A iculture and Forestry University, Fuzhou 350002. hina: .Institute of Soil and Fertilizer Sci
ences, Fujian Academy of Agricultural Sciences, Fuzhou 350013. China; 4. nstitute of Agricultural Ecology. Fujian Academy of Agricultural
Scienecs, Fuzhou 350013. China
Abstract: garicus brasiliensis is an edible and medicinal mushroom with a strong capability of cadmium accumulation, which may impact
[ood sceurity. Using a combination of Co-y ray and UV light, we previously bred a new A, brasiliensis strain named J by irradiating
mycelium of strain J r. Owing to its low Cd content and high stable yield, Jz was approved as a new variety named Fuji 77 by the Crop Variety
Approval ommiulce of Fujian Province in 2013. Using he/. brasiliensis strain J and ils mulan J as researeh malerials. the flees ol d
in diierent concentrations on mycelium growth rate and yields. Cd accumulation and conlens of three amino acids in mycelium, and ruiting
bodies of Jn and J were analyzed by adding different concentrations of Cd into the cullure medium lo reveal thc differenees in Cd uplake
tolerance, and physiological response mechanisms between J, and Jx. The results showed that mycelium growth rate and yields of 1 and Jn
increased first and then declined with the increase of d concentration. which indicated that Cd of lower concentrations could promote the
growth of mycelium and increase the yields. Under the same concentration of d, d contents in both mycelium and fruiting body of r were
significanlly lower than those of J, which indicated thal z was a lower Cd accumulative strain. In addition. Where was a signilicant positive
收稿日期:2017-01-13
作者简介:刘朋虎(1982-),男,山东莘县人,副研究员,主要从事食用菌重金属污染与遗传、育种等方间的研究。E-mai:phil1982@163.com
通信作者:翁伯琦R-mail:engomi(16.3.com
基金项目:福炷省自然科学基金项日(201501077);福建省中青年教师教育科研项日(JA13108);福建省菌草生态产业协同创新中心攻关项日
(K80DN8002-ICXTGG16)
Project supported: The Natural Science Foundation of Fu an rovince( 2015J01077): Foundation for Young and Middle-aged Teacher of Fujian Education
Department(JA 108); Key Subject of Cooperation Innovation Center of JL NCAO Ecolo