高压液相色谱法同时测定食品中抗坏血酸与异抗坏血酸的含量.pdf

第4卷第1期
食品安全质量检测学报
Vol 4 No
2013年2月
Journal of Food Salcly and Quality
lcb..2013
高压液相色谱法同时测定食品中抗
坏血酸与异抗坏血酸的含量
张清,李刚12
(1.吉林农业大学食品与科学工程学院,长春130118
2.吉林省产品质量监督检验院,长春130022)
摘要:目的建立食品中同时测定抗坏血酸和异抗坏血酸含量的高压液相色谱检測方法。方法用乙腈
0.1%6磷酸水溶液(90:10.vの作流动相和提取液,以 Venus HIIRC色谱柱(250mmx4.6mm,5umn,100)分离
抗坏血酸和异抗坏血酸。结果抗坏血酸的平均回收率为9661%,异抗坏血酸的平均回收率为96.53%异抗坏
血酸和抗坏血酸最低检出浓度分别为035ugmL和0.42g/mL。结论该方法准确、灵敏、精密度高,适用
于食品中抗坏血酸与异抗坏血酸的测定。
关键词:抗坏血酸;异抗坏血酸:高压液相色谱; Venus HIILC色谱柱
Simultaneous determination of ascorbic acid and isoascorbic acid in food by
high pressure liquid chromatography
ZHANG Qing, LI Gang-"2
1. College o/Food Sciece d ec nlogy, ili c m n e, Changchun 130118, ina
2. ilin Product Ouality Supervision and Inspection Research Institute, Changchun 130022, China)
ABSTRACT: Objective To establish a high pressure liquid chtomatography(HPI C) melhod for
simultancous dctermination of thc contents of ascorbic acid and isoascorbic acid in food Methods Ascorbic
acid and isoascorbic acid were scparated using a Venusil HILIC colum(250 mmx4. 6 mm, 5 um, 100 A)
laking acetonitrile-0.% phosphoric acid solution (90: 10, M/v)as the mobile phase and extractant. Results
The average recoveries of ascorbic acid and isoascorbic acid were 96.61%0 and.53%. And their minimum
detectable conccntration was 0.35 ug/ML and 0.42 Hg/ML Conclusion Thc method is accurate, sensitive and
of precision, and can be used for the delermination of ascorbic acid and isoascorbic acid in food
KEY WORDS: ascorbic acid; isoascorbic acid; high pressure liquid chromatography; Venusil IIII, IC colum
菜中?,人体不能自身合成,必须靠摄取获得,严重
缺乏时可引起坏血病。异抗坏血酸(I)-抗坏血酸)
抗坏血酸,即L-抗坏血酸,又称AA,是重要的也叫iso-AA,是Ⅰ-抗坏血酸的光学异构体,常用做
人体营养成分。抗坏血酸广泛存在于新鲜的水果,蔬食品抗氧化剂,具有保鲜保质的作用。它也可参与人
基金项目:吉质监标函(2011235
supported by
y Jilin Province 1bureau of Quality and Techology Supervision Iocal Standards I etter(2011235)
通讯作者:李刚,教授,主要研究方向为食品分析检测。T-mail:ly56k(yahoo.com,cn
Corresponding author II Gang, Irofessor Jilin Province roduct uality upcvision Test Insti. No 1088, Nanhu Road hanchun 130022, China
E-mail:lg56k(@yahoo.com.cn