高效液相色谱法测定熟肉制品中的刚果红.pdf

第6卷第2期
食品安全质量检测学报
Vol 6 No. 2
2015年2月
Journal of Food Salcly and Quality
lcb..2015
高效液相色谱法测定熟肉制品中的刚果红
窦彩云”,马育松,康占省,葛世辉
(河北出入境检验检疫局技术中心,石家庄050051)
摘要:目的建立高效液相色谱测定熟肉制品中刚果红的检测方法。方法样品经石油醚去除脂肪,以氨水
乙醇(20:80,体积比)溶液提取、乙酸调节pH至中性,以水定容、离心。经/ ORBAX SB-Aq色谱柱(4.6mm×250
mm,5um)分离,甲醇-20mmol.乙酸铵为流动相,梯度洗脱,检测波长500nm、,外标法定量。结果刚果红在
0.1~10ugmL浓度范围内线性关系良好,相关系数大于0.99,添加回收率在82.5%~90.7%之间,相对标准
偏差为在2.26%~7.29%之间,方法检出限为0.02mgkg。结论该方法简便、快捷,回收率和重现性好,适用
于熟肉制品中刚果红的测定。
关键词:刚果红;熟肉制品;高效液相色谱法
Determination of Congo red in cooked meat products by high performance
liquid chromatography
DOU Cai-yun, MA Yu-song, KANG Zhan-sheng, GE Shi-hui
(echnicalcener of Hebei Ent -xit Inspection and uarantine Bureall, Shijiazhang 050051, China)
ABSTRACT: Objcctive To establish a method of determination of Congo red in cooked meat products b
high pcrformance liquid chromato raphy. Methods The fat of the samples was removed with petroleum, and
then the samples were extracted by ammonia-ethanol(20: 80, ) PH was adjusted to 7.0-7.5 with acetic acid
and diluted with water to constant volume and then by centrifugation. Congo red was separated on a/OR3AX
SB-AG( 4.6 mm x250 mm, 5 Mm )with mcthanol-20 mmol /L ammonium acetate as mobile phase by gradicnt
elution, and detected al 500 nm, the external standard method was used [or quantitative analysis. Results
Congo red has a good linear relalionship in range ol 0.1-10 H/ml(>0.999). The recoveries were between
82. 5%90.7%, with relative standard deviations(RSD) of 2.26%-7.29. The limit of detection was 0.02
mg/kg. Conclusion This mcthod is simple, feasible, with good recovery and reproducibility, it is suitable for
delection of Congo red in cooked meal products
KEY WORDS: Congo red; cooked meat; high performance liquid chromatography
剂使用标准》叫中不包含该物质。2013年12月16
日欧洲化学总署(ECHA)正式发布 REACHI法规第十
刚果红是一种典型的联苯胺类偶氮染料、一般批高度关注物质清单,新增的7种物质中就包括刚果
用作医学检验、生物染色剂及化学指示剂,非食用色红,对其分类定义为致癌2。但刚果红由于其着色力
素,不允许加入到食品中,《GB2760-2011食品添加强、价格低廉,有不法商贩将其添加到熟肉中为其染
通讯作者:窦彩云,硕士,工程师,主要研究方向为理化检验。T-mai:cydou2013163.com
Corresponding author: DOU Cai-yun, Enginccr, Technical enter of Hcbci Entry-exit Inspcction and Quarantinc Burca, No 318, Hcping
Road,XinhuaDistrict,shijiazhuang050051,china.E-mail:cydou2013(163.com