抑制化学发光法检测甲萘威的研究.pdf

第23卷第11期
化学研究与应用
Vol 23.No 11
2011年11月
Chemical Research and Application
Nov.,2011
文章编号:1004-1656(2011)11-145605
抑制化学发光法检测甲萘威的研究
王翠霞,贾风燕,刘振波,刘永明
(烟台大学化学生物理工学院,山东烟台264005)
摘要;研究发现甲萘威对鲁米诺-过氧化氢-叶绿素铜钠体系的化学发光具有强烈抑制作用,由此建立了流动
注射-抑制化学发光检测甲滎威的新方法,并利用荧光猝灭法探讨了甲萘威对该体系化学发光的抑制机理。
实验表明叶绿素铜钠对甲莠威的荧光猝灭过程是静态猝灭过程,二者以摩尔比1:1结合,形成稳定的络合
物,平衡常数为5.43x10-L?mol'(298K),叶绿素铜钠-甲萘威络合物的形成降低了叶绿素铜钠对鲁米诺-过
氧化氢化学发光体系的催化作用。探讨了化学发光的最佳实验条件,在最佳条件下,甲萘威浓度在0.25
10.0pg?L范围内与化学发光信号值呈线性关系,检出限为0.08g?L2,回收率为9%6.7%~106.0%。
关键词:流动注射;化学发光;鲁米诺;叶绿素铜钠;甲萘威;荧光猝灭
中图分类号:0657.39文献标识码:A
Study on the inhibited chemiluminescence determination of carbar
WANG Cui-xia, JA Feng-yan, FU Zhen-bo, LIU Yong-ming
( School of Chemical and Biological cience and Engineering, Yantai University, Yantai 264005, China)
Abstract: It has been observed that carbaryl inhibited the Chemiluminescence(CL) signal of the luminol hydrogen peroxide-sodium
copper chlorophyllin system. In this work, we use fluorescence quenching spectra for investigating the mechanism of carbaryl inhibi
ting the CL system. The results indicated that the binding reaction between carbaryl and sodium copper chlorophyllin is a single stat
ic quenching prcess, so m coer chlorophyllin stony bins with cabar at the molar ratio of 1: 1 and the binding equilibrium
constant K s 5. 4310L m at 298 l, e stable complex was ommed etween sodium copper chlorophyllinand car
ay, therefore the catalysis ability of sodium copper chorophyllin for CL reaction was reduced. The influence factors on the FI-CL
sysem were investigae. The intensity of e L was a ar union of e concenration of carbaryl in the range. 25-10. w
L and the detection limit was 0.08 ug. L. The recovery of standard addition is 96.7%-106.0%
Key wor flow-in ec on luminescence; mind dium copper horoph illin; carbaryl; fluorescence quenching
甲恭威( carbaryl),化学名称1-汬基-N-甲基氨内分泌,损害生殖系统,影响人类健康]。随着人
基甲酸酯,为高毒广谱性杀虫剂,具有触杀和胃毒民生活水平的提高,食品安全日益受到社会的关
作用,广泛用于果树、蔬菜、药材、茶等经济作物的注,食品中残留农药的检测也越来越受到重视,加
害虫防治。甲禁威对人畜高毒,酸性土壤中不入WTYO后,我国农产品由于农药残留过高而遭拒
易降解,植物能通过根、茎、叶吸收传导,在叶缘积收、退货、索赔、终止合同等事件时有发生。因此
累,因处理不当食用被甲萘威污染的蔬菜中毒事控制农药残留量,已经成为提高我国农产品在国
件时有发生2。极低浓度的甲萘威就能干扰人体内外市场竞争力和关系人民身体健康的大事。研
收稿日期:201101-27;修回日期:1201105-10
联系人简介:刘永明(1962-),男,教授,主要从事仪器分析教学与科研工作。Emial:liuyongming1009126.com