小麦和玉米中脱氧雪腐镰刀菌烯醇与雪腐镰刀菌烯醇的免疫亲和净化-高效液相色谱检测方法研究.pdf

第5卷第12期
食品安全质量检测学报
Vol 5 No 12
2014年12月
Journal of Food Salcly and Quality
Dce..2014
小麦和玉米中脱氧雪腐镰刀菌烯醇与雪腐镰刀菌
烯醇的免疫亲和净化一高效液相色谱检测方法研究
吴振兴",鲍蕾,静平,门爱军2,庞国兴3,赵华梅
(1.山东出入境检验检疫局检验检疫技术中心,青岛266002.山东出入境检验检疫局,青岛266000
3.青岛出入境检验检疫局、青岛26000
摘要:目的建立小麦和玉米中脱氧雪腐镰刀菌烯醇与雪腐镰刀菌烯醇的免疫亲和净化-高效液相色谱检测
方法。方法样品经纯水提取后、用免疫亲和柱净化,经甲醇洗脱,在C18色谱柱上等度洗脱分离,采用紫外检
测器检测。结果标准曲线在0.1-2.0mg/kg范围内线性良好。小麦基质中脱氧雪腐镰刀菌烯醇的回收率为
72.89%-110.1%、精密度为3.6%-10.8%,实验室内 Horrat值为0.24-0.48;玉米中脱氧雪腐镰刀菌烯醇的回收
率为72.29090.6%,精密度为1.2%~5.2%,实验室内 Borat值为0.07~0.31。小麦基质中雪腐鐮刀菌烯醇的
回收率为58.9%~100.4%,精密度为3.6%~1.3%,实验室内 Horrat值为0.23-0.63:;玉米中雪腐镰刀菌烯醇
的回收率为56.9%-91.9%,精密度为2.5%-7.8%,实验室内 Horral值为0.1~-0.43。结论该方法具有灵敏
度高、重现性好、操作简便、准确可靠等特点,适用于小麦和玉米中脱氧雪腐镰刀菌烯醇与雪腐镰刀菌烯醇的
测定
关键词:脱氧雪腐镰刀菌烯醇;雪腐镰刀菌烯醇:免疫亲和浄化;高效液相色谱法
Determination and analysis of deoxynivalenol and nivalenol in wheat and
corn using immunoaffinity column cleanup and high performance
liquid chromatography
WU Zhen-xing", BAO Lei, JING Ping MEN Ai-jun, PANG Guo-xing, ZHAO Hua-meil
(1. echmica/ Center of Shandong Enin-exil Inspection and uramine Bure, Qingda 266000, Chin
2. Shandong Entry-exir inspection and Ouaramtine Bueau, Qingdao 266000, Chin
3. ingdao Enirry-exit Inspec/ionand Oarumlime Buren, ingdao 266000, China)
ABSTRACT: Objcctive To establish a method for the determination of deoxynivalenol(DON) and
nivalenol (NT V in wheat and corn by using immunoaffinity colum cleanup and high performance liquid
chromatography (TPT. C). Methods The sample was extracted with water, then passed through the
immunoaffinity column containing antibodies spe ific for I(N and NIV. The DON and NIV were sequentially
cluted by 0.5 ML methanol and 1.5 IL acetonitrile, and then were scparatcd on Cis colum. The DON and NV
were detected by IIPI. C/V Results The linear ranges of aflatoxin were 0.1-2.0 mg/kg, and the recoveries o
基金项目:国家质检总局科技计划项目(20121K174、20061K105)、山东检验检疫局科技计划项目(SK201363)
Fund: Supportd by the cicntitic and Technological Project of the Gcncral Administration of Quality Supervision, Inspection and Quarantinc of
the Pcoplcs Republic of China(20121K174, 2006K105)and the cicntific and Technological Projcct of Shandong Entry-exit Inspcetion and
Ouarantine ureau (Sk201363
通讯作者:吴振兴,高级工程师,主要研究方向为生物毒素检测。T-mail:howo(a126.com
Corresponding author: WU Zhen-xing, Senior Enginccr, Tochnical cntc of Shandong Entry-exit Inspection and Quarantinc Burcau
Qingdao266000,China.E-mail:zhxwoo(e126.com