酶联免疫吸附法测定小麦中脱氧雪腐镰刀菌烯醇.pdf

第4卷第1期
食品安全质量检测学报
Vol 4 No
2013年2月
Journal of Food Salcly and Quality
lcb..2013
晦联免疫吸附法测定小麦中脱氧雪腐镰刀菌烯醇
肖娟,辛苑,冯莎,栾先国,李凤琴2,赵丽
(1.山东大学公共卫生学院,济南250012
2.中国疾病预防控制中心营养与食品安全所,北京100052)
摘要:目的建立小麦中脱氧雪腐镰刀菌烯醇( deoxynivalenol,D)ON)含量测定的酶联免疫吸附(I.ISA)分析
方法。方法选取山东省4地区小麦样品80份,用不同溶剂对小麦中的D(N进行提取后构建小麦中D(ON的
HISA检测方法,根据国家标准对检测结果进行评价。结果选用84%的乙腈水溶液作为提取溶剂,其平均回
收率可达100.2%,较为理想。该HISA方法检测小麦中1)N,线性苑围是5-5000ngg,回归方程y=
0.0419X+0.0222相关系数r0.9868,样品中D)ON含量为5.27~3639.38ngg,中位数为79.25ngg,在所检测
的80份样品中,有4份超出了国家规定的上限标准(1000ngg)276份符合国家规定,合格率为95%。结论该
方法灵敏、快捷,适用于小麦中脱氧雪腐镰刀菌烯醇的检测。
关键词:脱氧雪腐镰刀菌烯醇:小麦;酶联免疫吸附法
Determination of deoxynivalenol in wheat by enzyme-linked
Immunosorbent assay
XIAO Juan, XIN Yuan, FENG Sha, LUAN Xian-guo LI Feng-qin, ZHAO Lil
Schoo/ofpublic Health, Shandong [,, Jinan 250012, China
2. Institute o/Na/rition and Food hygiene, Chinese enter for Disease Conro nd Preention, Beijing 100052, hin)
ABSTRACT: Objcctive To explore a method of enyme-linked immunosorbent assay(T TS) for determi-
nation of deoxynivalenol( DON)in wheat. Methods A total of 80 wheat samples collected from 4 regions o
Shandong province were cxtracted by different solvents, detected by T. ISA and evaluated according to na-
tional standard. Results Optimal DON cxtraction was obtaincd when 84%0 acetonitrile was used in the extrac-
tion agent which could guarantce the mean cxtraction rate of 100.2%0. Ihe mcthod showcd a good linearity ovcr
the range of 5-5000 ng/g for deoxynivalenol and the regression cquation was Y =0.0419X+0.0222 with
7=0.9868. The covent of deoxynivalenol in wheal was 5.27-3639.38 ng/g with median of 79.25 ngg. The con-
centration of 4 samples among the totals was higher than the DON Lolerance limitation o[ 1000 ng/g, and the
qualified rate was 95%. Conclusion The method is accurate and eflicient and il is suitable for the determina
Lion O[ DON in wheal
KEY WORDS: deoxynivalenol; wheat; cnzyme-linkcd immunosorbent assay
脱氧雪腐镰刀菌烯醇( deoxynivalenol,DON)是B和粉红蠊刀菌的某些菌株产生,在许多谷物如小麦
类单端孢霉烯族化合物中的一种,主要由禾谷镰刀菌大麦和玉米中普遍存在。其化学性能稳定,具有较强
通讯作者:赵丽,副教授,博士,主要研究方向为微生物检验。F-mail: dlrhl(a sdu.edu.cn
Corresponding author ZHAO Ii, Assocate Pro ssor School of Public Hath, Shandon rsity, No 44, Wcnhuaxi Road, Jinan 250012, China
E-mail:jingdaping(agmail.com